Concept

Trypsin

Related publications (32)

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

EPFL2023

Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms

Grégoire Michielin

In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...

EPFL2021

PUS3-related disorder: Report of a novel patient and delineation of the phenotypic spectrum

Alessandro Borghesi

PUS3 encodes the pseudouridylate synthase 3, an enzyme catalyzing the formation of tRNA pseudouridine, which plays a critical role in tRNA structure, function, and stability. Biallelic pathogenic variants of PUS3 have been previously associated with severe ...

WILEY2021

Potential bioavailability of inorganic compounds from Tire and Road Wear Particles to fish in simulated gastric and intestinal fluids

Florian Frédéric Vincent Breider, Benoît Jean Dominique Ferrari, Thibault Béranger Masset

The potential impact of Tire and Road Wear Particles (TRWP) on aquatic organisms has recently gained attention since the occurrence of TRWP in the aquatic environment has been demonstrated in surface water and sediments of numerous regions. However, data r ...

2020

The role of the polyglutamine and N-terminal domains in regulating the aggregation and structural properties of Huntingtin Exon 1

Sophie Vieweg

Huntington Disease (HD) is caused by a CAG repeat expansion in the huntingtin gene leading to the formation of mutant Huntingtin protein (Htt) with an expanded polyglutamine (polyQ) domain (>36Q). Generation of short N-terminal Htt fragments by proteolysis ...

EPFL2017

Middle-down approaches for mass spectrometry-based protein identification and characterization

Kristina Srzentic

Mass spectrometry (MS) has emerged over the last two decades as the analytical technique of choice in systems-level protein studies, known as proteomics. Two are the MS-based approaches generally applied to proteomics: bottom-up (BU), which relies on the p ...

EPFL2016

Amino-functionalized macroporous silica for efficient tryptic digestion in acidic solutions

Hubert Girault, Liang Qiao, Jingjing Wan

Amino-functionalized macroporous silica foam (NH2-MOSF) has been developed as a host reactor to realize highly efficient proteolysis in acidic solutions where normal tryptic reactions cannot occur. The digestion protocol consists simply of adding the funct ...

Wiley-Blackwell2013

Phage selection of cyclic peptide antagonists with increased stability toward intestinal proteases

Christian Heinis, Vanessa Baeriswyl

The oral delivery of protein and peptide drugs is limited by their proteolytic degradation and the poor absorption across the intestinal epithelia. In this work, we exposed a phage library of small bicyclic peptides (

Oxford University Press2013

Mechanistic Studies Related to Nickel-Catalyzed Alkyl-Alkyl Cross Coupling Reactions

Jan Breitenfeld

Cross-coupling reactions of non-activated alkyl halides are potentially useful chemical transformations. At the same time, however, they are challenging due to a series of unproductive side reactions. Recently, significant progress has been made to overcom ...

EPFL2013

Validation of an in vitro digestive system for studying macronutrient decomposition in humans

Martinus Gijs, Guy Vergères, Flurina Schwander, Reto Portmann

The digestive process transforms nutrients and bioactive compounds contained in food to physiologically active compounds. In vitro digestion systems have proven to be powerful tools for understanding and monitoring the complex transformation processes that ...

2012

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