Erythrocyte fragility

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Biology
Cell biology
Blood

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In vitro-transfusional model for red-blood-cell study: the advantage of lowering hematocrit

Gerardo Turcatti, Manon Sandra Bardyn, David Crettaz, Michel Prudent

Background - Thequalityof red blood cells (RBCs) stored in red cell concentrates (RCCs) is influenced by processing, storage and donor characteristics, and can have a clinical impact on transfused patients. To evaluate RBC properties and their potential im ...

Simtipro Srl2023

Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules

Gerardo Turcatti, Benjamin Rappaz, David Crettaz, Michel Prudent, Manon Sandra Bardyn

An increase of oxygen saturation within blood bags and metabolic dysregulation occur during storage of red blood cells (RBCs). It leads to the gradual exhaustion of RBC antioxidant protective system and, consequently, to a deleterious state of oxidative st ...

2021

Inhibition of influenza virus activity by the bovine seminal plasma protein PDC-109

Christian Sieben

A number of viruses causing sexually transmissible diseases are transmitted via mammalian seminal plasma. Several components of seminal plasma have been shown to influence those viruses and their physiological impact. To unravel whether components of semin ...

SPRINGER2019

Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging

Gerardo Turcatti, Benjamin Rappaz, Pierre Marquet, Michel Prudent, Manon Sandra Bardyn

We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell me ...

2018

Automated tracking of temporal displacements of a red blood cell obtained by time-lapse digital holographic microscopy

Benjamin Rappaz

Red blood cell (RBC) phase images that are numerically reconstructed by digital holographic microscopy (DHM) can describe the cell structure and dynamics information beneficial for a quantitative analysis of RBCs. However, RBCs investigated with time-lapse ...

Optical Soc Amer2016

The motion of a deforming capsule through a corner

Lailai Zhu

A three-dimensional deformable capsule convected through a square duct with a corner is studied via numerical simulations. We develop an accelerated boundary integral implementation adapted to general geometries and boundary conditions. A global spectral m ...

Cambridge University Press2015

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Francesco Stellacci, Paulo Henrique Jacob Silva, Ahmet Bekdemir, Randy Patrick Carney, Ye Yang

Erythrocytes are attractive as potential cell-based drug carriers because of their abundance and long life-span in vivo. Existing methods for loading drug cargos into erythrocytes include hypotonic treatments, electroporation, and covalent attachment onto ...

Royal Society of Chemistry2015

Erythrocyte-binding therapeutics

Jeffrey Alan Hubbell, Stephane Kontos, Karen Dane

Peptides that specifically bind erythrocytes are described. These are provided as peptidic ligands having sequences that specifically bind, or as antibodies or fragments thereof that provide specific binding, to erythrocytes. The peptides may be prepared a ...

2013

Automated quantitative analysis of 3D morphology and mean corpuscular hemoglobin in human red blood cells stored in different periods

Pierre Marquet, Daniel Boss

Quantitative phase (QP) images of red blood cells (RBCs), which are obtained by off-axis digital holographic microscopy, can provide quantitative information about three-dimensional (3D) morphology of human RBCs and the characteristic properties such as me ...

Optical Soc Amer2013

Functional and Structural Imaging with Optical Coherence Microscopy : Principles & Applications

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

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