Chemical defense is a strategy employed by many organisms to avoid consumption by producing toxic or repellent metabolites or chemical warnings which incite defensive behavioral changes. The production of defensive chemicals occurs in plants, fungi, and bacteria, as well as invertebrate and vertebrate animals. The class of chemicals produced by organisms that are considered defensive may be considered in a strict sense to only apply to those aiding an organism in escaping herbivory or predation. However, the distinction between types of chemical interaction is subjective and defensive chemicals may also be considered to protect against reduced fitness by pests, parasites, and competitors. Repellent rather than toxic metabolites are allomones, a sub category signaling metabolites known as semiochemicals. Many chemicals used for defensive purposes are secondary metabolites derived from primary metabolites which serve a physiological purpose in the organism. Secondary metabolites produced by plants are consumed and sequestered by a variety of arthropods and, in turn, toxins found in some amphibians, snakes, and even birds can be traced back to arthropod prey. There are a variety of special cases for considering mammalian antipredatory adaptations as chemical defenses as well. Bacteria of the genera Chromobacterium, Janthinobacterium, and Pseudoalteromonas produce a toxic secondary metabolite, violacein, to deter protozoan predation. Violacein is released when bacteria are consumed, killing the protozoan. Another bacteria, Pseudomonas aeruginosa, aggregates into quorum sensing biofilms which may aid the coordinated release of toxins to protect against predation by protozoans. Flagellates were allowed to grow and were present in a biofilm of P. aeruginosa grown for three days, but no flagellates were detected after seven days. This suggests that concentrated and coordinated release of extracellular toxins by biofilms has a greater effect than unicellular excretions.

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