Publications related to Molecular cloning

Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate

Witold Waldemar Kilarski

The first reports about successfully expressed recombinant proteins with the use of a baculovirus vector were published over 30 years ago. Despite the long time of refining this expression system, early problems with the production of baculovirus-derived s ...

Academic Press Inc Elsevier Science2015

A single epidermal stem cell strategy for safe ex vivo gene therapy

Graham Knott, Jacques Rougemont, Yann Barrandon, Ariane Rochat, Andrea Zaffalon, Nicolas Grasset, Stéphanie Danièle Hélène Droz-Georget, Sara Benmohammed, Emmanuelle Savioz-Dayer, Alessandra Recchia

There is a widespread agreement from patient and professional organisations alike that the safety of stem cell therapeutics is of paramount importance, particularly for ex vivo autologous gene therapy. Yet current technology makes it difficult to thoroughl ...

Wiley Open Access2015

Improved biocompatibility of polyethylenimine (PEI) as a gene carrier by conjugating urocanic acid: In vitro and in vivo

Jeffrey Alan Hubbell, Irina Krier, Jong Eun Ihm

Polyethylenimine (PEI) is a promising gene carrier among polymeric vectors because of its high transfection efficiency, buffering capacity, which is responsible for its escape from endosomes, facility of modification, reasonable price and wide range of mol ...

Polymer Soc Korea2015

Identification of single nucleotides in MoS2 nanopores

Aleksandra Radenovic, Andras Kis, Dumitru Dumcenco, Ke Liu, Jiandong Feng, Roman Bulushev, Sergey Khlybov

The size of the sensing region in solid-state nanopores is determined by the size of the pore and the thickness of the pore membrane, so ultrathin membranes such as graphene and single-layer molybdenum disulphide could potentially offer the necessary spati ...

Nature Publishing Group2015

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Patrick Aebischer, Matthias Lütolf, Bernard Schneider, Steffen Cosson, Aurélien Lathuilière

The rapid increase in the number of approved therapeutic proteins, including recombinant antibodies, for diseases necessitating chronic treatments raises the question of the overall costs imposed on healthcare systems. It is therefore important to investig ...

Elsevier2014

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Liang Qiao, Fernando Cortes Salazar, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

Virus-free transient protein production in Sf9 cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen

A method for virus-free transient gene expression from suspension-adapted Sf9 insect cells was developed with the gene of interest being expressed from a plasmid carrying the homologous region 5 enhancer (hr5) and the immediate early 1 (ie1) promoter from ...

Elsevier2014

Composition of the DNA-uptake complex of Vibrio cholerae

Melanie Blokesch, Lisa Christina Metzger

Natural competence for transformation is a developmental program that allows certain bacteria to take up free extracellular DNA from the environment and integrate this DNA into their genome. Thereby, natural transformation acts as mode of horizontal gene t ...

2014

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Molecular cloning

Study of transposon-mediated cell pool and cell line generation in CHO cells

Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate

A single epidermal stem cell strategy for safe ex vivo gene therapy

Improved biocompatibility of polyethylenimine (PEI) as a gene carrier by conjugating urocanic acid: In vitro and in vivo

Identification of single nucleotides in MoS2 nanopores

Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Virus-free transient protein production in Sf9 cells

Composition of the DNA-uptake complex of Vibrio cholerae

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Chat with Graph Search

Improved biocompatibility of polyethylenimine (PEI) as a gene carrier by conjugating urocanic acid: In vitro and in vivo

Composition of the DNA-uptake complex of Vibrio cholerae

Study of transposon-mediated cell pool and cell line generation in CHO cells

Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells

Virus-free transient protein production in Sf9 cells

A single epidermal stem cell strategy for safe ex vivo gene therapy

Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate

Identification of single nucleotides in MoS2 nanopores

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels