Concept

Molecular cloning

Related publications (174)

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sagar Shashidhar Manoli

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

A Genetic Basis for Altered Sexual Behavior in Mutant Female Mice

Denis Duboule, Jozsef Zakany

Although neural substrates of mammalian female mating behavior have been described [1, 2], the association between complex courtship activity and specific underlying mechanisms remains elusive [3]. We have isolated a mouse line that unexpectedly shows alte ...

Elsevier2012

Chemical macrocyclization of peptides fused to antibody Fc fragments

Christian Heinis, David Hacker, Laure Menin, Alessandro Angelini, Philippe Diderich, Julia Morales Sanfrutos, Sarah Thurnheer

To extend the plasma half-life of a bicyclic peptide antagonist, we chose to link it to the Fc fragment of the long-lived serum protein IgG1. Instead of chemically conjugating the entire bicyclic peptide, we recombinantly expressed its peptide moiety as a ...

Amer Chemical Soc2012

A simple high-yielding process for transient gene expression in CHO cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak

Here we describe a simplified method for transient gene expression (TGE) in suspension-adapted Chinese hamster ovary (CHO) cells using polyethylenimine (PEI) for DNA delivery. Both the transfection and production phases of the bioprocess were performed at ...

Elsevier2011

Large-Scale Transfection of Mammalian Cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Carine Meerschman

The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneim ...

Humana Press2011

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

Florian Maria Wurm, David Hacker, Lucia Baldi Unser

Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable ...

Wiley-Blackwell2011

Investigation of the role of PICALM on amyloid metabolism in a transgenic mouse model of Alzheimer’s disease

Dominique Rubi

Recently, two large independent genome-wide association studies have provided compelling evidence that polymorphisms at the phosphatidylinositol-binding clathrin assembly protein (PICALM) locus are linked to Alzheimer’s disease (AD). At the cellular level, ...

2011

Two strategies to improve transient gene expression in HEK293E and/or CHO-DG44 cells

Guillaume Lüthi

Transient gene expression (TGE) is based on episomal DNA expression. This technique is used for the production of recombinant proteins that can be used as therapeutics. TGE is for example extremely useful for shortening the time between candidate proteins ...

2011

Structure-function analysis points to a polynucleotide-accommodating groove essential for APOBEC3A restriction activities

Didier Trono, Priscilla Turelli, Amalio Telenti, Shyam Sunder Peddi, Olivier Michielin

Members of the human APOBEC3 family of editing enzymes can inhibit various mobile genetic elements. APOBEC3A (A3A) can block the retrotransposon LINE-1 and the parvovirus adeno-associated virus type 2 (AAV-2) but does not inhibit retroviruses. In contrast, ...

American Society for Microbiology2010

Leaching and transformability of transgenic DNA in unsaturated soil columns

Unsaturated soil columns were used to examine the transport of the plasmid pLEPO1 and plant DNA (transplastomic tobacco DNA), both carrying an antibiotic resistance gene (aadA gene), and the capacity of bacteria to incorporate the gene in their genome afte ...

2010

Graph Chatbot

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.