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Monoclonal IgA was produced under serum free conditions by a murine hybridoma cell line (ZAC3) in a hollow fiber, a continuous stirred tank and a fluidized bed reactor. Differences in the antibody adsorption to DEAE chromatog. matrixes, an essential step i ...
We studied the effects of iron supplied as transferrin-bound iron and iron supplied as non-transferrin iron on transferrin receptor expression by human cell lines. Defined conditions of iron supply were represented by (i) 5 microg/ml of iron-saturated tran ...
Neutralizing antibodies are necessary and sufficient for protection against infection with vesicular stomatitis virus (VSV). The in vitro neutralization capacities and in vivo protective capacities of a panel of immunoglobulin G monoclonal antibodies to th ...
The cytotoxic effect of different concentrations of titanium particles on osteoblasts was studied in vitro. It was found that the viability of the osteoblasts was inversely proportional to the particle concentration. Phagocytosis of particles by the osteob ...
A new simple concept for a miniaturized monolayer trough is described. The overall monolayer area in the expanded state is approx. 150 mm2 and can be reduced by a factor of 2. The surface area is a function of the shape of the meniscus formed by the subpha ...
Mounting evidence from in vitro experiments indicates that lactate is an efficient energy substrate for neurons and that it may significantly contribute to maintain synaptic transmission, particularly during periods of intense activity. Since lactate does ...
Continuous cultures of a hybridoma cell line (Zac3) were performed with 2 14C-labeled substrates: glucose and glutamine. A simplified network was used to describe cell metab. This simple network gave a good idea of the main fluxes into the cell, esp. for c ...
CD80 and CD86 (B7-1 and B7-2) are the ligands on antigen-presenting cells (APCs) which bind CD28 and deliver the costimulatory signals necessary for T cell activation. The reasons for the existence of two CD28 binding molecules are not well understood. We ...
Batch cultures of a mouse/mouse hybridoma cell line secreting a dimeric IgA were performed in combination with a membrane process for the continuous removal of ammonia from the culture medium. The latter involves hydrophobic porous membranes with an assocd ...
Anti- V.cholerae monoclonal IgA antibodies have been produced using a hybridoma cell line (Zac3) grown in RPMI medium contg. 10% Fetal calf serum (FCS) and a completely defined protein- free medium. The latter has been optimized with respect to amino acid, ...
