Concept

Real-time polymerase chain reaction

Related publications (150)

Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer

Martinus Gijs, Raphaël Etienne Jean Trouillon, Maryse Fiche, Huu Tuan Nguyen, Seiya Matsuoka

Fluorescence in situ hybridization (FISH) is one of the recommended techniques for human epidermal growth factor receptor 2 (HER2) status assessment on cancer tissues. Here we develop microfluidics-assisted FISH (MA-FISH), in which hybridization of the FIS ...

Nature Publishing Group2017

DNA barcoding of formalin‑fixed aquatic oligochaetes for biomonitoring

Régis Lionel Vivien

Background: Oligochaetes are valuable bioindicators of the quality of watercourse and lake sediments. The morphological identification of aquatic oligochaetes is difficult, prompting the development of new molecular oligochaete indices based on DNA barcodi ...

2016

Contribution of hepatic cytochrome CYP1A and metallothionein mRNA abundance to biomonitoring-A case study with European flounder (Platichthys flesus) from the Gulf of Gdansk

Kristin Schirmer

The aim of the research was to explore the contribution of hepatic cytochrome CYP1A and metallothionein (MT) mRNA expression to biological effect monitoring. The study was conducted in the European flounder (Platichthys flesus) from the Gulf of Gdansk. mRN ...

Elsevier Science Inc2016

Label-Free Electrochemical Detection of MicroRNA-122 in Real Samples by Graphene Modified Disposable Electrodes

Tugba Kilic

In the present work, an electrochemical nucleic acid biosensor has been designed for the purpose of detection of miR-122 in real samples, i.e cell lysates. The fabrication of the biosensor has been done first by immobilization of complemantary anti-miR-122 ...

Electrochemical Soc Inc2016

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Zuzana Kadlecova, Sowmya Balasubramanian

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...

Elsevier2015

Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs

Paolo De Los Rios, Pierre Goloubinoff, Vishal Sood, Andrija Finka

Classic semiquantitative proteomic methods have shown that all organisms respond to a mild heat shock by an apparent massive accumulation of a small set of proteins, named heat-shock proteins (HSPs) and a concomitant slowing down in the synthesis of the ot ...

Springer2015

Real-Time Label-Free Direct Electronic Monitoring of Topoisomerase Enzyme Binding Kinetics on Graphene

Klaus Kern, Kannan Balasubramanian

Mono layer graphene field-effect sensors operating in liquid have been widely deployed for detecting a range of analyte species often under equilibrium conditions. Here we report on the real-time detection of the binding kinetics of the essential human enz ...

Amer Chemical Soc2015

Gene expression mapping of histone deacetylases and co-factors, and correlation with survival time and 1H-HRMAS metabolomic profile in human gliomas

Johan Auwerx

Primary brain tumors are presently classified based on imaging and histopathological techniques, which remains unsatisfaying. We profiled here by quantitative real time PCR (qRT-PCR) the transcripts of eighteen histone deacetylases (HDACs) and a subset of ...

2015

Quantitative Estimation of Biological Cell Surface Receptors by Segmenting Conventional Fluorescence Microscopy Images

Giovanni De Micheli, Sandro Carrara, Danilo Demarchi, Paolo Silacci, Julien Michel Ghaye, Chiara Succa, Guy Vergères

State-of-the-art techniques for measuring and monitoring gene level expression rely on messenger RNA (mRNA) extraction and quantification, usually based on the concept of reverse transcription polymerase chain reaction. In this paper, we take adva ...

Ieee2014

Hybridization chain reaction performed on a metal surface as a means of signal amplification in SPR and electrochemical biosensors

Carlotta Guiducci, Fabio Mario Spiga, Giampaolo Zuccheri

A more specific and intense signal is desirable for most kinds of biosensors for biomedical or environmental applications, and it is especially so for label-free biosensors. In this paper, we show that hybridization chain reaction (HCR) can be exploited fo ...

Elsevier2014

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