Concept

Genetic engineering techniques

Related publications (81)

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Control of growth and gut maturation by &ITHoxD&IT genes and the associated lncRNA &ITHaglr&IT

Denis Duboule, Jozsef Zakany, Fabrice Daniel Darbellay, Anamaria Necsulea

During embryonic development, Hox genes participate in the building of a functional digestive system in metazoans, and genetic conditions involving these genes lead to important, sometimes lethal,growth retardation. Recently, this phenotype was obtained af ...

Proceedings of the National Academy of Sciences2017

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

Florian Maria Wurm, David Hacker, Sowmya Balasubramanian

Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...

American Chemical Society2016

The mammary gland is the only organ to undergo most of its development after birth and therefore particularly attractive for studying developmental processes. In the mouse, powerful tissue recombination techniques are available that can be elegantly combin ...

2016

Joint mouse-human phenome-wide association to test gene function and disease risk

Johan Auwerx, Zhi Li, Evan Graehl Williams, Xu Wang, Virginija Jovaisaite

Phenome-wide association is a novel reverse genetic strategy to analyze genome-to-phenome relations in human clinical cohorts. Here we test this approach using a large murine population segregating for similar to 5 million sequence variants, and we compare ...

Nature Publishing Group2016

Genetic targeting of chemical indicators in vivo

Kai Johnsson, Luc Reymond, Antonino Asaro

Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labe ...

Nature Publishing Group2015

Horizontal gene transfer (HGT) is widespread in bacteria and archaea and plays a significant role in prokaryotic evolution. One mode of HGT is natural competence for genetic transformation, which allows the organism to take up free DNA from its surrounding ...

Humana Press2015

The goal of this thesis was to evaluate the use of artificial transposon systems for the generation of recombinant cell pools and cell lines with Chinese hamster ovary (CHO) cells as the production host. Transposons are naturally occurring genetic elements ...

EPFL2015

Ex vivo gene therapy using retrievable encapsulated cellular implants is an effective strategy for the local and/or chronic delivery of therapeutic proteins. In particular, it is considered an innovative approach to modulate the activity of the immune syst ...

MDPI2015

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Zuzana Kadlecova, Sowmya Balasubramanian

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...

Elsevier2015

Genetic engineering of cell lines using lentiviral vectors to achieve antibody secretion following encapsulated implantation

Patrick Aebischer, Bernard Schneider, Marc Moniatte, Aurélien Lathuilière

The controlled delivery of antibodies by immunoisolated bioimplants containing genetically engineered cells is an attractive and safe approach for chronic treatments. To reach therapeutic antibody levels there is a need to generate renewable cell lines, wh ...

Elsevier2014