Concept
Mutagenesis (molecular biology technique)
Related publications (16)
HaloTag-Based Tools for Live-Cell Imaging and Elucidation of Organellar Redox Biology
Redox homeostasis is a key factor in maintaining cellular function and health. The main determinant of the intracellular redox potential is ubiquitous glutathione (GSH) together with its oxidized dimer (GSSG). Importantly, both redox equilibrium and GSH ho ...
EPFL2024A Microfluidic Platform Enables Large-Scale Single-Cell Screening to Identify Genes Involved in Bacterial Persistence
Persistent cells, representing a small fraction of a bacterial population, are refractory to antibiotic therapy. It is a phenotypic transient state. The underlying mechanisms of persistence remain largely unknown, but even divergent hypotheses agree on a f ...
EPFL2017Study of transposon-mediated cell pool and cell line generation in CHO cells
The goal of this thesis was to evaluate the use of artificial transposon systems for the generation of recombinant cell pools and cell lines with Chinese hamster ovary (CHO) cells as the production host. Transposons are naturally occurring genetic elements ...
EPFL2015TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrate ...
2012Single-Cell Analysis of Mycobacterial Persistence Using Microfabricated Tools
Bacterial cells behave as individuals despite being genetically identical and subject to the same environment. Although the underlying mechanisms of cellular individuality are not well understood, temporal variation of gene expression and protein levels in ...
EPFL2012Exploring the Capabilities of Nucleic Acid Polymerases by Use of Directed Evolution
Kai Johnsson, Petra Tafelmeyer
A review. Directed evolution consists of repetitive cycles of random mutagenesis of the protein/peptide sequence followed by screening or selection of candidates with desired properties. Many different approaches are used to introduce mutations into a gene ...
Wiley2004