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Concept
Guanosine diphosphate
Summary
Guanosine diphosphate, abbreviated GDP, is a nucleoside diphosphate. It is an ester of pyrophosphoric acid with the nucleoside guanosine. GDP consists of a pyrophosphate group, a pentose sugar ribose, and the nucleobase guanine.
GDP is the product of GTP dephosphorylation by GTPases, e.g., the G-proteins that are involved in signal transduction.
GDP is converted into GTP with the help of pyruvate kinase and phosphoenolpyruvate.
The hydrolysis of GTP to GDP is facilitated by GTPase enzymes, which utilize a conserved active site motif known as the GTPase-activating protein (GAP). Initially, a water molecule is coordinated by the active site residues of the GTPase enzyme. The water molecule attacks the γ-phosphate of GTP, leading to the formation of a pentavalent transition state. This transition state is stabilized by interactions with the active site residues, including conserved catalytic residues. As a result, the γ-phosphate is cleaved, and inorganic phosphate (Pi) is released. This step also causes a conformational change in the enzyme that promotes the release of GDP.
GDP is involved in intracellular signaling processes functioning as a critical regulator in the activity of GTPases. GTPases act as molecular switches, cycling between an active GTP-bound state and an inactive GDP-bound state. The interconversion between GDP and GTP is tightly controlled and serves as a molecular timer for signal transduction pathways. When an extracellular signal triggers the activation of a G-protein coupled receptor (GPCR), the associated G-protein exchanges its bound GDP for GTP, leading to a conformational change and activation of downstream signaling cascades. This activation can stimulate a variety of cellular responses, including modulation of gene expression, cytoskeletal rearrangements, and regulation of enzymatic activities. The hydrolysis of GTP to GDP by the GTPase activity of the G-protein restoring the inactive state, thus terminates the signaling event.
Official source
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