Vero cells are a lineage of cells used in cell cultures. The 'Vero' lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species). The lineage was developed on 27 March 1962, by Yasumura and Kawakita at the Chiba University in Chiba, Japan. The original cell line was named Vero after an abbreviation of verda reno, which means 'green kidney' in Esperanto, while vero itself means 'truth' in Esperanto.
The Vero cell lineage is continuous and aneuploid, meaning that it has an abnormal number of chromosomes. A continuous cell lineage can be replicated through many cycles of division and not become senescent.
Vero cells are interferon-deficient; unlike normal mammalian cells, they do not secrete interferon alpha or beta when infected by viruses. However, they still have the Interferon-alpha/beta receptor, so they respond normally when recombinant interferon is added to their culture media.
The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014. Chromosome 12 of Vero cells has a homozygous ~9-Mb deletion, causing the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitors CDKN2A and CDKN2B in the genome. Although African green monkeys were previously classified as Cercopithecus aethiops, they have been placed within the genus Chlorocebus, which includes several species. The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus.
Vero cells are used for many purposes, including:
screening for the toxin of Escherichia coli, first named "Vero toxin" after this cell line, and later called "Shiga-like toxin" due to its similarity to Shiga toxin isolated from Shigella dysenteriae
as host cells for growing viruses; for example, to measure replication in the presence or absence of a research pharmaceutical, the testing for the presence of rabies virus, or the growth of viral stocks for research purposes.
This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.
This laboratory-training course is designed to give students a comprehensive insight into laboratory research techniques in the field of biotechnology and pharmaceutical biotechnology with practices e
The course focuses on the development and application of biotechnology-based approaches to human diseases. It provides current information on the engineering and pharmaceutical production of highly sp
Computer modelling is increasingly used to study dynamic phenomena in cell biology. This course shows how to identify common mathematical features in cell biological mechanisms, and become proficient
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.
Covers the basics of tissue engineering, laboratory introductions, recommended courses, autografting, allografting, FDA-approved tissue products, and current research challenges.
The present relates to a system for automated cell culture, the system comprising an inflow module, a cell culture plate and an outflow module, said inflow module being in fluid connection with the outflow module via the cell culture plate, the inflow modu ...
Micrometer sized capsules are often used as single entities for the encapsulation and release of active ingredients, for example in food, cosmetics, and drug delivery. Important parameters that determine the stability of capsules and the release of reagent ...
WILEY2023
, , ,
We present a new cell culture technology for large-scale mechanobiology studies capable of generating and applying optically controlled uniform compression on single cells in 3D. Mesenchymal stem cells (MSCs) are individually encapsulated inside an optical ...