Publications related to Ligation (molecular biology)

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

Melanie Blokesch, David William Adams, Alexandre Lemopoulos, Grazia Vizzarro

In response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialised defence systems that are often clustered together on genomic islands. The O1 El Tor strains of Vibrio cholerae resp ...

2024

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Christian Heinis, Xudong Kong, Vanessa Carle, Cristina Diaz Perlas, Kaycie Marie Butler

The success of phage display, used for developing target-specific binders based on peptides and proteins, depends on the size and diversity of the library screened, but generating large libraries of phage-encoded polypeptides remains challenging. New pepti ...

2020

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering

Christian Iseli, Nicolas Mermod, Thomas Junier, Samuel Neuenschwander, Ioannis Xenarios

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous r ...

2017

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sowmya Balasubramanian

Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to reduce the pDNA amou ...

American Chemical Society2015

Exploiting Dispersion-driven Aggregators as a Route to New One-dimensional Organic Nanowires

Riccardo Petraglia, Adrien Georges Nicolai, Hongguang Liu

The efficiency of charge carrier mobility in organic semiconductors is heavily dependent upon the long-range organization (i.e., morphology) and the local relative arrangement of the transporting molecules. Here, we exploit London dispersion forces as a de ...

Amer Chemical Soc2015

Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability

Julien Hiblot, Pierre Leblond

We describe here the construction of a new version of the Escherichia coli-Streptomyces shuttle cloning vector pIJ6902, which carries the original thiostrepton-inducible PtipA as well as the integrative and cloning systems, but with the E. coil low copy nu ...

Academic Press Inc Elsevier Science2015

Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation

Julien Dorier, Yannis Burnier, Fabrizio Benedetti, Andrzej Stasiak

Due to the helical structure of DNA the process of DNA replication is topologically complex. Freshly replicated DNA molecules are catenated with each other and are frequently knotted. For proper functioning of DNA it is necessary to remove all of these ent ...

Oxford University Press2015

Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling

Giovanni Dietler, Julien Dorier, Yannis Burnier, Fabrizio Benedetti, Andrzej Stasiak, Aleksandre Japaridze, Robert Sebastian Kwapich

DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated from ...

Oxford University Press2015

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Ligation (molecular biology)

Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

Exploiting Dispersion-driven Aggregators as a Route to New One-dimensional Organic Nanowires

Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability

Modeling and Engineering Proteins Thermostability

Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation

Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling

Graph Chatbot

Chat with Graph Search

Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

Modeling and Engineering Proteins Thermostability

Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation

Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

Exploiting Dispersion-driven Aggregators as a Route to New One-dimensional Organic Nanowires

Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability