Concept

Rolling circle replication

Related publications (32)

Modeling and Engineering Proteins Thermostability

Enzymes have evolved during millions of years to become efficient catalysts for specific biochemical reactions within a specific range of working conditions in the cellular environment. The activity of an enzyme is directly related to its folded structure ...

EPFL2015

Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation

Julien Dorier, Yannis Burnier, Fabrizio Benedetti, Andrzej Stasiak

Due to the helical structure of DNA the process of DNA replication is topologically complex. Freshly replicated DNA molecules are catenated with each other and are frequently knotted. For proper functioning of DNA it is necessary to remove all of these ent ...

Oxford University Press2015

Chromosome Organization and Replisome Dynamics in Mycobacterium smegmatis

John McKinney, Isabella Santi

Subcellular organization of the bacterial nucleoid and spatiotemporal dynamics of DNA replication and segregation have been studied intensively, but the functional link between these processes remains poorly understood. Here we use quantitative time-lapse ...

American Society for Microbiology2015

Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling

Giovanni Dietler, Julien Dorier, Yannis Burnier, Fabrizio Benedetti, Andrzej Stasiak, Aleksandre Japaridze, Robert Sebastian Kwapich

DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated from ...

Oxford University Press2015

Quantifying supercoiling-induced denaturation bubbles in DNA

Giovanni Dietler

In both eukaryotic and prokaryotic DNA sequences of 30-100 base-pairs rich in AT base-pairs have been identified at which the double helix preferentially unwinds. Such DNA unwinding elements are commonly associated with origins for DNA replication and tran ...

Royal Society of Chemistry2012

Preparation of nucleic acid templates for solid phase amplification

Gerardo Turcatti

The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a l ...

2010

DNA supercoiling and its role in DNA decatenation and unknotting

Andrzej Stasiak, Guillaume Witz

Chromosomal and plasmid DNA molecules in bacterial cells are maintained under torsional tension and are therefore supercoiled. With the exception of extreme thermophiles, supercoiling has a negative sign, which means that the torsional tension diminishes t ...

Oxford University Press2010

Interplay of DNA supercoiling and catenation during the segregation of sister duplexes

Andrzej Stasiak, Guillaume Witz, Pablo Hernandez

The discrete regulation of supercoiling, catenation and knotting by DNA topoisomerases is well documented both in vivo and in vitro, but the interplay between them is still poorly understood. Here we studied DNA catenanes of bacterial plasmids arising as a ...

Oxford University Press2009

Telomerase is essential to alleviate pif1-induced replication stress at telomeres

Joachim Lingner

Pif1, an evolutionarily conserved helicase, negatively regulates telomere length by removing telomerase from chromosome ends. Pif1 has also been implicated in DNA replication processes Such as Okazaki fragment maturation and replication fork pausing. We fi ...

2009

Preparation of templates for nucleic acid sequencing

Gerardo Turcatti

The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to ...

2009

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