Concept

Transgene

Related publications (133)

Tissue-specific transgenic and knockout mice

Analysis of genetically engineered mice is crucial for our understanding of the in vivo function of genes and proteins in the whole organism. This includes inactivation of a gene or the generation of specific mutations. The development of knockout and tran ...

2006

A beta 42-driven cerebral amyloidosis in transgenic mice reveals early and robust pathology

Tristan Bolmont

We have generated a novel transgenic mouse model on a C57BL/ 6J genetic background that coexpresses KM670/ 671NL mutated amyloid precursor protein and L166P mutated presenilin 1 under the control of a neuron- specific Thy1 promoter element ( APPPS1 mice). ...

Nature Publishing Group2006

Identification of evolutionarily conserved regulatory elements in the mouse Fgf8 locus

Friedrich Beermann, Philipp Bucher

The secreted signaling molecule fibroblast growth factor 8 (Fgf8) is an essential component of certain embryonic signaling centers including the mid-hindbrain (isthmic) organizer, the first branchial arch (BA1), and the apical ectodermal ridge (AER). In th ...

2006

Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice

Florian Maria Wurm

BACKGROUND: Recent advances in functional genomics, gene therapy, tissue engineering, drug discovery and biopharmaceuticals production have been fostered by precise small-molecule-mediated fine-tuning of desired transgenes. METHODS: Capitalizing on well-ev ...

2005

Harnessing HIV for therapy, basic research and biotechnology

Didier Trono

First described about a decade ago, lentiviral vectors ('lentivectors') have emerged as potent and versatile tools of gene transfer for basic and applied research and offer exciting perspectives for the field of gene therapy. In the clinic, HIV-based vecto ...

2005

Oncogenesis Following Delivery of a Nonprimate Lentiviral Gene Therapy Vector to Fetal and Neonatal Mice

Didier Trono

Gene therapy by use of integrating vectors carrying therapeutic transgene sequences offers the potential for a permanent cure of genetic diseases by stable vector insertion into the patients' chromosomes. However, three cases of T cell lymphoproliferative ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller, Philippe Pierre Girard

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker

The Gam1 protein of the avian CELO adenovirus activates transcription through inhibition of histone deacetylase 1 (HDAC1). We investigated the effect of Gam1 on both transient and stable transgene expression in Chinese hamster ovary (CHO) cells, one of the ...

2005

Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS

Patrick Aebischer, Jean-Charles Bensadoun, Sandrine Guillot

Mutations in Cu/Zn superoxide dismutase (encoded by SOD1), one of the causes of familial amyotrophic lateral sclerosis (ALS), lead to progressive death of motoneurons through a gain-of-function mechanism. RNA interference (RNAi) mediated by viral vectors a ...

2005

A simple and highly effective method for the stable transduction of uncultured porcine hepatocytes using lentiviral vector

Didier Trono

Gene therapy is an attractive approach for the treatment of a wide spectrum of liver diseases. Lentiviral vectors allow the stable integration of transgenes into the genome of nondividing differentiated cells including hepatocytes and could provide long-la ...

2005

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