Concept
Mesonephric duct
Related publications (18)
Coordinated Hoxd genes expression during embryogenesis is dependent on a strict disposition of genes and polarized regulators within the HoxD cluster
HOX transcription factors determine the identity of body regions along the rostro-caudal axis during bilaterian embryogenesis. In vertebrates Hox genes distinctively lie organized in dense clusters, each typically composed of a dozen paralogous transcripti ...
EPFL2018Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation
TAF4 (TATA-binding protein-associated factor 4) and its paralogue TAF4b are components of the TFIID core module. We inactivated the murine Taf4a gene to address Taf4 function during embryogenesis. Here we show that Taf4a(-/-) embryos survive until E9.5 whe ...
Nature Publishing Group2016Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sarah Thurnheer
Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfect ...
Elsevier2013An ancestral regulatory mechanism underlies Hoxd gene expression in both developing genitals and digits
Coordinated expression of HoxD cluster genes is crucial for the emergence and patterning of both developing digits and external genitalia. As a shared evolutionary origin has been proposed for these secondary structures, which may have accompanied the tran ...
EPFL2013Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sagar Shashidhar Manoli
Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...
2012A regulatory ‘landscape effect’ over the Hoxd cluster
Faithful expression of Hox genes in both time and space is essential for proper patterning of the primary body axis. Transgenic approaches in vertebrates have suggested that this collinear activation process is regulated in a largely gene cluster-autonomou ...
Elsevier2011