Concept

Outer membrane vesicles

Related publications (273)

Integrated Nanoreactor Systems: Triggering the Release and Mixing of Compounds Inside Single Vesicles

Horst Vogel, Pierre-Yves Bolinger, Dimitrios Stamou

We present a method that allows the on-demand release and mixing of zepto- to femtoliter vols. of solns. in the interior of vesicular nanoreactors. The reactors comprise a nested system of lipid vesicles, part of which release their cargo in the interior o ...

2004

Identification of bacterial muramyl dipeptide as activator of the NALP3/cryopyrin inflammasome

Etienne Meylan

Activation of caspase-1 and subsequent processing and secretion of the pro-inflammatory cytokine IL-1beta is triggered upon assembly of the inflammasome complex. It is generally believed that bacterial lipopolysaccharides (LPS) are activators of the inflam ...

Elsevier2004

Electrokinetic transport of PAH-degrading bacteria in model aquifers and soil

An investigation of the mobility, viability, and activity of polycyclic aromatic hydrocarbon (PAH) degrading bacteria in an electric field is presented. Bench-scale model aquifers were used to test electrophoresis and electroosmosis as potential mechanisms ...

2004

Bacterial subversion of lipid rafts

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

Bacteria rely on numerous basic cellular functions of their target cells to reach successful infection. The recent discovery that the plasma membrane contains specialized microdomains, called lipid rafts, with many specific functions but in particular with ...

Elsevier2004

Investigating cellular signalling reactions by micro- and nanotechnology

Horst Vogel

Classically, cellular signalling is investigated measuring different optical or elec. properties of either single cells or microliter vols. of suspensions of live cells. Here we show how ligand binding to cell surface receptors and subsequent signalling re ...

2004

Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method

Geoffrey Bodenhausen, Fabien Ferrage

The translational diffusion coeff. of an integral membrane protein/surfactant complex has been measured using a novel pulsed field gradient NMR method. In this new approach, the information about the localization of the mols. is temporarily stored in the f ...

2003

Fabrication of functional structures on thin silicon nitride membranes

Jürgen Brugger

A process to fabricate functional polysilicon structures above large (4 x 4 mm(2)) thin (200 nm), very flat LPCVD silicon rich nitride membranes was developed. Key features of this fabrication process are the use of low-stress LPCVD silicon nitride, sacrif ...

2003

Multiaperture sample positioning and analysis system

Horst Vogel

Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivs., and mixts. thereof, for elec. and/or optical anal., esp. relating to the presence and/or activity of ion channels. [on SciFinder (R)] ...

(Switz.).2003

Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic beta-cells

Didier Trono, Romain Zufferey, Alessandra Calabrese

We have generated novel lentiviral vectors to integrate various connexin cDNAs into primary, non-dividing cells. We have used these vectors to test whether proper control of insulin secretion depends on a specific connexin isoform and/or on its level of ex ...

2003

Morphological and molecular heterogeneity in release sites of single neurons

Harald Hirling, Juan-Carlos Sarria, Xin Wang

We have previously shown that labelling intensities for synaptic proteins vary strongly among synaptic boutons. Here we addressed the questions as to whether there are heterogeneous levels of integral membrane synaptic vesicle proteins at distinct active r ...

2003

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