Concept

Directed differentiation

Related publications (92)

Engineering of extracellular matrix proteins : Integrin specific fibronectin fragment for mesenchymal stem cells differentiation and design of fibronectin-laminin fusion protein

[...] This project focuses on specific α5β1 integrin (α5β1) activation, because its crucial roles in differentiation and in adhesion have already been studied. The project is articulated in two related parts. The first and main one is dedicated to specific ...

2008

The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype

Peter Frey, Jeffrey Alan Hubbell

The formation of scar tissue due to dedifferentiation of smooth muscle cells (SMCs) is one of the major issues faced when engineering bladder tissue. Furthermore, cell sources for regenerating the SMC layer are also limiting. Here we explore if human mesen ...

2008

Isolation of hematopoietic stem cells from mouse embryonic stem cells

Olaia Maria Naveiras Torres-Quiroga

This unit describes a protocol for the isolation of cells from murine embryonic stem cells with hematopoietic stem cell activity, defined by the ability to reconstitute, long term, multiple lineages of the hematopoietic system of lethally irradiated mice. ...

Wiley2008

Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin

Fabien Kuttler, Ilaria Malanchi, Stéphane Duboux

The canonical Wnt signaling pathway plays key roles in stem-cell maintenance, progenitor cell expansion, and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either beta-catenin or gamma-catenin with lymphoid enhance ...

2008

Neural commitment of embryonic stem cells: molecules, pathways and potential for cell therapy

David Michael Suter

The study of neuronal differentiation of embryonic stem cells has raised major interest over recent years. It allows a better understanding of fundamental aspects of neurogenesis and, at the same time, the generation of neurons as tools for various applica ...

Wiley-Blackwell2008

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Boris Hinz, Lara Buscemi Estefanell, Pierre Jean Arnould Wipff

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008

The cdx-hox pathway in hematopoietic stem cell formation from embryonic stem cells

Olaia Maria Naveiras Torres-Quiroga

Embryonic stem cells (ESCs) differentiated in vitro will yield a multitude of hematopoietic derivatives, yet progenitors displaying true stem cell activity remain difficult to obtain. Possible causes are a biased differentiation to primitive yolk sac-type ...

Wiley-Blackwell2007

Method of enhancing proliferation and/or hematopoietic differentiation of stem cells

Olaia Maria Naveiras Torres-Quiroga

The present invention provides methods for inducing differentiation of a stem cell, such as an embryonic stem cell, into a hematopoietic stem cell, by expressing a cdx gene and/or a hox gene. The method is useful for generating expanded populations of hema ...

2007

Induction of cardiogenesis in embryonic stem cells via downregulation of Notch1 signaling

Freddy Radtke

Embryonic stem cells represent an attractive source of cardiomyocytes for cell-replacement therapies. However, before embryonic stem cells can be successfully used for the treatment of cardiac diseases, the precise molecular mechanisms that underlie their ...

2006

Stem cells and their niche: a matter of fate

Olaia Maria Naveiras Torres-Quiroga

Embryonic stem cells provide an in vitro model for developmental biologists to study cell fate decisions during ontogenesis, while somatic stem cells allow physiologists to understand tissue homeostasis in the adult. The behavior of stem cells is dependent ...

Springer Verlag2006

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