Publications related to Two-dimensional gel electrophoresis

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Hubert Girault, Liang Qiao, Mohammad Reza Pourhaghighi

An advanced approach is developed in this work for simultaneous on-line separation and digestion of proteins by combining the Off-Gel isoelectric focusing (IEF) and enzymatic nanoreactor enhanced proteolysis. The nanoreactor was prepared by preloading tryp ...

Elsevier2015

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Liang Qiao, Fernando Cortes Salazar, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

A four-component organogel based on orthogonal chemical interactions

Kay Severin, Kurt Schenk, Nicolas Luisier

A thermoresponsive organogel was obtained by orthogonal assembly of four compounds using dynamic covalent boronate ester and imine bonds, as well as dative boron-nitrogen bonds. It is shown that the gel state can be disrupted or reinforced by chemicals whi ...

Royal Society of Chemistry2014

Coupling Isoelectric Focusing Gel Electrophoresis to Mass Spectrometry by Electrostatic Spray Ionisation

Hubert Girault, Liang Qiao, Elena Tobolkina

Gel electrophoresis has been used for decades as a high-resolution separation technique for proteins and protein isomers, but limited in the coupling with MS because of low throughput and poor automaticity compared with LC-MS. In this work, we have develop ...

American Chemical Society2013

Sedimentation of macroscopic rigid knots and its relation to gel electrophoretic mobility of DNA knots

Giovanni Dietler, Cédric Weber, Andrzej Stasiak, Mathias Carlen

We address the general question of the extent to which the hydrodynamic behaviour of microscopic freely fluctuating objects can be reproduced by macrosopic rigid objects. In particular, we compare the sedimentation speeds of knotted DNA molecules undergoin ...

Nature Publishing Group2013

The molecular mechanisms of antimetastatic ruthenium compounds explored through DIGE proteomics

Paul Joseph Dyson, Angela Casini

DIGE (difference in gel electrophoresis) proteomics is exploited here to gain insight into the molecular mechanisms of two established ruthenium-based antimetastatic agents, namely trans-[tetrachloro (DMSO) (imidazole)ruthenate(III)] (NAMI-A) and [Ru(eta(6 ...

Elsevier Science Inc2013

Orthogonality of Two-Dimensional Separations Based on Conditional Entropy

Hubert Girault, Mohammad Reza Pourhaghighi, Mohammad Karzand

A new approach to assess the orthogonality of two-dimensional (2-D) separation systems based on conditional entropy is developed. It considers the quantitative distribution of peaks in the entire separation space such that the orthogonality obtained is ind ...

American Chemical Society2011

Gel form of ECM-scaffold prepared from bovine Nucleus Pulposus.

Philippe Abdel Sayed, Alizée Jéléna Grimm

Decellularized tissues composed of extracellular matrix (ECM) and used as biologic scaffolds, have been shown to be useful for the repair of a variety of tissues. The clinical utility of such scaffolds for the tissue engineering of Nucleus Pulposus (NP) mi ...

2011

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Two-dimensional gel electrophoresis

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

A four-component organogel based on orthogonal chemical interactions

New analytical tools combining gel electrophoresis and mass spectrometry

Coupling Isoelectric Focusing Gel Electrophoresis to Mass Spectrometry by Electrostatic Spray Ionisation

Sedimentation of macroscopic rigid knots and its relation to gel electrophoretic mobility of DNA knots

The molecular mechanisms of antimetastatic ruthenium compounds explored through DIGE proteomics

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Orthogonality of Two-Dimensional Separations Based on Conditional Entropy

Gel form of ECM-scaffold prepared from bovine Nucleus Pulposus.

Graph Chatbot

Chat with Graph Search

Gel form of ECM-scaffold prepared from bovine Nucleus Pulposus.

Coupling Isoelectric Focusing Gel Electrophoresis to Mass Spectrometry by Electrostatic Spray Ionisation

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Orthogonality of Two-Dimensional Separations Based on Conditional Entropy

Sedimentation of macroscopic rigid knots and its relation to gel electrophoretic mobility of DNA knots

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

A four-component organogel based on orthogonal chemical interactions

New analytical tools combining gel electrophoresis and mass spectrometry

The molecular mechanisms of antimetastatic ruthenium compounds explored through DIGE proteomics