Concept

Two-dimensional gel electrophoresis

Related publications (55)

Off-Gel[TM] electrophoresis

The Off-Gel™ technology is an isoelectric focusing (IEF) fractionation method which enables the purification of one or several proteins in a solution placed on an immobilised pH gradient (IPG) gel. The present thesis is devoted to its characterisation and ...

EPFL2004

Impacts of heavy metal contamination and phytoremediation on a microbial community during a twelve-month microcosm experiment

Fabienne Gremion

The effects of heavy metals and phytoextraction practices on a soil microbial Community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulcseens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bact ...

Blackwell Publishing Ltd, Oxford2004

Protein fractionation in a multicompartment device using Off-Gel(TM) isoelectric focusing

Hubert Girault, Joël Stéphane Rossier

A new protein fractionation technique based on off-gel isoelectric focusing (IEF) is presented, where the proteins are separated according to their isoelectric point (pI) in a multiwell device with the advantage to be directly recovered in solution for fur ...

Wiley-Blackwell2003

Molecular ecological analysis of porcine ileal microbiota responses to antimicrobial growth promoters

Bart Deplancke

Cultivation-independent microbial molecular ecology approaches were used to examine the effects of antibiotic growth promoters on the pig ileal microbiota. Five-week-old barrows were fitted with a simple T-cannula at the distal ileum. Three diets meeting o ...

2003

Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns

Pierre Rossi, François Gillet

Technical developments in molecular biology have found extensive applications in the field of microbial ecology. Among these techniques, fingerprinting methods such as denaturing gel electrophoresis (DGE, including the three options: DGGE, TGGE and TTGE) h ...

2002

Selective growth of mucolytic bacteria including Clostridium perfringens in a neonatal piglet model of total parenteral nutrition

Bart Deplancke

BACKGROUND: Compromised barrier function and intestinal inflammation are common complications of total parenteral nutrition (TPN). OBJECTIVE: We tested the hypothesis that the lack of enteral nutrients in TPN might select commensal or pathogenic bacteria t ...

2002

Analysis of glycosyl phosphatidylinositol-anchored proteins by two-dimensional gel electrophoresis

Françoise Gisou van der Goot Grunberg

The aim of this study was to characterize mammalian glycosyl phosphatidylinositol (GPI)-anchored proteins y two-dimensional gel electrophoresis using immobilized pH gradients. Analysis was performed on detergent-resistant membrane fractions of baby hamster ...

2000

Comparative characterization of a wild type and transmembrane domain-deleted fatty acid amide hydrolase: identification of the transmembrane domain as a site for oligomerization

Hilal Lashuel

Fatty acid amide hydrolase (FAAH) is an integral membrane protein responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. Analysis of FAAH's primary sequence re ...

American Chemical Society1998

Identification of bacteriophage T4 prereplicative proteins on two-dimensional polyacrylamide gels

John McKinney

Bacteriophage T4 makes a large number of prereplicative proteins, which are involved in directing the transition from host to phage functions, in producing the new T4 DNA, and in regulating transcriptional shifts. We have used two-dimensional gel electroph ...

American Society for Microbiology1994

Export of mRNA from microinjected nuclei of Xenopus laevis oocytes

Lukas Kühn

Export of mRNA from the nucleus to the cytoplasm was studied in mature Xenopus laevis oocytes. In vitro transcribed, capped 32P-labeled mRNA was microinjected into nuclei, and its appearance in the cytoplasm measured by counting radioactivity or by RNA ext ...

1992

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