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Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell. Due to the high fl ...
Plasmid DNA was originally produced in small amounts for genetic modification for protein production purposes. However since the rapid advances in gene therapy and DNA vaccines, plasmid DNA became a therapeutic product. The use of non-viral vectors for saf ...
An on-line pH monitoring method based on mid-infrared spectroscopy relevant to bioprocesses is presented. This approach is non-invasive and does not require the addition of indicators or dyes, since it relies on the analysis of species of common buffers us ...
The estn. of the intracellular fluxes of mammalian cells using only the mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is undetd. Either addnl. exptl. flux data or addnl. theor. ...
A partial least-squares calibration model, relating mid-IR spectral features with fructose, ethanol, acetate, gluconacetan, phosphate and ammonium concns. has been designed to monitor and control cultivations of Gluconacetobacter xylinus and prodn. of gluc ...
This thesis aimed at developing new, simple methods of calibration for on-line monitoring of bioprocesses by Fourier-transform mid-infrared spectroscopy (FTIR). The conventional calibration approach implies the preparation and measurement of a large number ...
The bioconversion of L-phenylalanine to 2-phenylethanol by Saccharomyces cerevisiae in fed-batch expts. has shown that concns. of 2-phenylethanol of >2.9 g/L have a neg. impact on the oxidative capacity of the yeast. Without tight control on ethanol prodn. ...
A FIA method has been developed and fully characterized for the simultaneous detection of ammonia and glutamine in culture media. Ammonia detection is based upon a chem. method and does not require an electrode. This species diffuses across a hydrophobic p ...