Recirculating aquaculture systems (RAS) are used in home aquaria and for fish production where water exchange is limited and the use of biofiltration is required to reduce ammonia toxicity. Other types of filtration and environmental control are often also necessary to maintain clean water and provide a suitable habitat for fish. The main benefit of RAS is the ability to reduce the need for fresh, clean water while still maintaining a healthy environment for fish. To be operated economically commercial RAS must have high fish stocking densities, and many researchers are currently conducting studies to determine if RAS is a viable form of intensive aquaculture. A series of treatment processes is utilized to maintain water quality in intensive fish farming operations. These steps are often done in order or sometimes in tandem. After leaving the vessel holding fish the water is first treated for solids before entering a biofilter to convert ammonia, next degassing and oxygenation occur, often followed by heating/cooling and sterilization. Each of these processes can be completed by using a variety of different methods and equipment, but regardless all must take place to ensure a healthy environment that maximizes fish growth and health. Biofiltration All RAS relies on biofiltration to convert ammonia (NH4+ and NH3) excreted by the fish into nitrate. Ammonia is a waste product of fish metabolism and high concentrations (>.02 mg/L) are toxic to most finfish. Nitrifying bacteria are chemoautotrophs that convert ammonia into nitrite then nitrate. A biofilter provides a substrate for the bacterial community, which results in thick biofilm growing within the filter. Water is pumped through the filter, and ammonia is utilized by the bacteria for energy. Nitrate is less toxic than ammonia (>100 mg/L), and can be removed by a denitrifying biofilter or by water replacement. Stable environmental conditions and regular maintenance are required to ensure the biofilter is operating efficiently.

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