Concept
Nuclease
Related publications (36)
Export of a protein into the outer membrane of Escherichia coli K12: Stable incorporation of the OmpA protein requires less than 193 amino-terminal amino-acid residues
The cloned ompA gene encoding the major outer membrane protein OmpA of Escherichia coli has been shortened in vitro by exonuclease digestion from the end corresponding to the CO2H terminus of the protein. Nine derivatives were identified which still posses ...
1982Genetic and physical characterization of lambda transducing phages (lambda frdA) containing the fumarate reductase gene of Escherichia coli K12
Two types of fumarate reductase transducing phages, lambda frdA, carrying the wild-type frdA gene but differing in the orientation of a R.HindIII fragment of bacterial DNA were isolated from populations of recombinant transducing phages by their ability to ...
1980Analysis of DNA double- and single-strand breaks by two dimensional electrophoresis: action of micrococcal nuclease on chromatin and DNA, and degradation in vivo of lens fiber chromatin
We describe a novel system for two dimensional electrophoresis at neutral and alkaline pH for determining the double-stranded and single-stranded lengths of DNA. With this system we analysed the mode of micrococcal nuclease digestion of DNA in cellular and ...
1980Amplification of fumarate reductase synthesis with lambdafrdA transducing phages and orientation of frdA gene expression
Thermoinducible lysis-defective derivatives of λfrdA phages (λG1F and λG40F) carrying the fumarate marate reductase gene of Escherichia coli, inserted in each of two possible orientations, were used to amplify fumarate reductase synthesis and study the aer ...
1980Mobility of histones on the chromosome of simian virus 40
Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA cov ...
1978Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease
S(1) nuclease, the single-strand specific nuclease from Aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (SV40) DNA to generate unit length linear duplex molecules with intact single strands. But circul ...
1973