Concept

Orbitrap

Related publications (32)

Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody-Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry

Yury Tsybin, Natalia Gasilova, Laure Menin, Anton Kozhinov, Konstantin Nagornov

The therapeutic efficacy and pharmacokinetics of antibody-drug conjugates (ADCs) in general, and antibody-oligonucleotide conjugates (AOCs) in particular, depend on the drug-to-antibody ratio (DAR) distribution and average value. The DAR is considered a cr ...

AMER CHEMICAL SOC2021

Online Aerosol Chemical Characterization by Extractive Electrospray Ionization-Ultrahigh-Resolution Mass Spectrometry (EESI-Orbitrap)

Julia Schmale, Imad El Haddad

Current mass spectrometry techniques for the online measurement of organic aerosol (OA) composition are subjected to either thermal/ionization-induced artifacts or limited mass resolving power, hindering accurate molecular characterization. Here, we combin ...

AMER CHEMICAL SOC2020

Multiplexed Middle-Down Mass Spectrometry as a Method for Revealing Light and Heavy Chain Connectivity in a Monoclonal Antibody

Yury Tsybin, Natalia Gasilova, Laure Menin, Anton Kozhinov, Konstantin Nagornov, Luca Fornelli, Kristina Srzentic, Daniel Ayoub

Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) or middle-down (MD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, ...

AMER CHEMICAL SOC2018

Identification of Isomeric Ephedrines by Cold Ion UV Spectroscopy: Toward Practical Implementation

Vladimir Kopysov

Ephedrine and pseudoephedrine are stimulant drugs whose use is prohibited in athletic competition by the World Anti Doping Agency (WADA) at very different threshold doping violation concentrations. We use a recently developed universal approach that integr ...

2017

Top-down analysis of immunoglobulin G isotypes 1 and 2 with electron transfer dissociation on a high-field Orbitrap mass spectrometer

Yury Tsybin, Luca Fornelli, Daniel Ayoub

The increasing importance of immunoglobulins G (IgGs) as biotherapeutics calls for improved structural characterization methods designed for these large (similar to 150 kDa) macromolecules. Analysis workflows have to be rapid, robust, and require minimal s ...

Elsevier Science Bv2017

Novel Approaches in Cold Ion Spectroscopy for Structural Characterization of Biomolecules in the Gas Phase

Vladimir Kopysov

Mass spectrometry is a powerful analytical technique, which has become invaluable across a wide range of scientific fields, such as "omics" sciences (e.g., proteomics), doping control and forensic sciences, drug development, environmental analysis, geologi ...

EPFL2016

Colors for Molecular Masses: Fusion of Spectroscopy and Mass Spectrometry for Identification of Biomolecules

Vladimir Kopysov, Oleg Boyarkine

We present an approach that integrates ultraviolet (UV) photofragmentation spectroscopy of cold ions with high-resolution Orbitrap mass spectrometry (MS) and uses mathematical analysis of the recorded 2D data arrays for structural identification of biomole ...

Amer Chemical Soc2015

Ion coalescence in Fourier transform mass spectrometry: should we worry about this in shotgun proteomics?

Luca Fornelli

Coupling of motion of the ion clouds with close m/z values is a well-established phenomenon for ion-trapping mass analyzers. In Fourier transform ion cyclotron resonance mass spectrometry it is known as ion coalescence. Recently, ion coalescence was demons ...

Im Publications2015

Time-dependent frequency of ion motion in Fourier transform mass spectrometry

Yury Tsybin

Recent experimental and theoretical findings suggest that the concept of time-dependent instantaneous frequency(IF) is required to comprehensively describe ion motion and mass spectra generation in Fourier transform mass spectrometry (FTMS). Here, we deriv ...

Elsevier Science Bv2015

Numerical experiments with MALDI Imaging data

Pierre Vandergheynst, Mohammad Golbabaee

This article does not present new mathematical results, it solely aims at discussing some numerical experiments with MALDI Imaging data. However, these experiments are based on and could not be done without the mathematical results obtained in the UNLocX p ...

Springer2014

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