Concept

DNA polymerase I

Related publications (42)

Poly(ADP-ribose) polymerase-2 [corrected] controls adipocyte differentiation and adipose tissue function through the regulation of the activity of the retinoid X receptor/peroxisome proliferator-activated receptor-gamma [corrected] heterodimer

The peroxisome proliferator-activated receptor-gamma (PPARgamma, NR1C3) in complex with the retinoid X receptor (RXR) plays a central role in white adipose tissue (WAT) differentiation and function, regulating the expression of key WAT proteins. In this re ...

2007

BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

Gerardo Turcatti

The tricarboxylate reagent benzene-1,3,5-triacetic acid (BTA) was used to attach 5'-aminated DNA primers and templates on an aminosilanized glass surface for subsequent generation of DNA colonies by in situ solid-phase amplification. We have characterized ...

Oxford University Press2006

Microelectrodes on a silicon chip for label-free capacitive DNA sensing

Carlotta Guiducci

This paper presents the experimental characterization of two-terminal microfabricated capacitors for microarrays with an electrical sensing of label-free deoxyribonucleic acid (DNA). So far, such a concept has been demonstrated only in experimental setups ...

2006

Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms

Gerardo Turcatti

Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated f ...

Oxford University Press2000

Methods of nucleic acid amplification and sequencing

Gerardo Turcatti

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5 ...

2000

The Mycobacterium leprae genome: systematic sequence analysis identifies key catabolic enzymes, ATP-dependent transport systems and a novel polA locus associated with genomic variability

Stewart Cole

In the framework of the mycobacterial genome sequencing project, a continuous 37,049 bp sequence from the Mycobacterium leprae chromosome has been determined. Computer analysis revealed 10 complete open reading frames, and nine of their products show simil ...

1995

A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

Stewart Cole

The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers ...

1993

Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae

Joachim Lingner

Poly(A) polymerase is essential for the maturation of messenger RNA, adding tracts of adenosine residues to the 3' end of precursor RNA generated by endonucleolytic cleavage. This mechanism of mRNA 3' processing seems to be similar in yeast and in higher e ...

1991

Overexpression and purification of a biologically active rifampicin-resistant beta subunit of Escherichia coli RNA polymerase

John McKinney

The gene rpoB (rifD 18), which encodes rifampicin-resistant beta subunit of Escherichia coli RNA polymerase, has been placed on an overexpression plasmid under the control of bacteriophage T7 promoter. Induction of the T7 RNA polymerase gene in the host ce ...

1987

Preferential repair of N-acetoxy-acetylaminofluorene lesions in the nuclease-hypersensitive region of simian virus 40

Peter Martin Beard

We exposed simian virus 40-infected CV-1 monkey cells to the carcinogen N-acetoxy-acetylaminofluorene and monitored the removal of lesions from cellular DNA and from various regions of viral DNA. Exposure to 5.5 microM 3H-labeled carcinogen produced 20-80 ...

1987

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