Concept

Okazaki fragments

Related publications (46)

Development of a new reporter system for the detection of protein-protein interactions in living cells

Split-protein sensors have become an important tool for the analysis of protein-protein interactions in living cells. In general, two interacting proteins are expressed as fusion proteins with a pair of inactive fragments of a reporter enzyme. Interaction- ...

EPFL2004

A gateway-compatible yeast one-hybrid system

Bart Deplancke

Since the advent of microarrays, vast amounts of gene expression data have been generated. However, these microarray data fail to reveal the transcription regulatory mechanisms that underlie differential gene expression, because the identity of the respons ...

2004

Microfluidic Device for Nucleic Acid Fragmentation by Shear Force

Nico de Rooij

A microfluidic device for random fragmentation of DNA or RNA based on the application of a hydrodynamic shear force to these biomolecules is presented. Different from conventional fragmentation instruments, the microchip-based implementation allows molecul ...

Springer2002

Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy

Henning Paul-Julius Stahlberg, Andreas Engel

Secretins, a superfamily of multimeric outer membrane proteins, mediate the transport of large macromolecules across the outer membrane of Gramnegative bacteria. Limited proteolysis of secretin PulD from the Klebsiella oxytoca pullulanase secretion pathway ...

Wiley-Blackwell2000

Transparent Replication for Fault Tolerance in Distributed Ada 95

In this paper we present the foundations of Rapids ("Replicated Ada Partitions In Distributed Systems"), an implementaion of the PCS supporting the transparent replication of partitions in distributed Ada 95 using semi-active replication. The inherently no ...

ACM Press1999

Return of lymphatic function after flap transfer for acute lymphedema

Melody Swartz

OBJECTIVE: The goals of this work were to develop animal models of lymphedema and tissue flap transfer, and to observe physiologic changes in lymphatic function that occur in these models over time, both systemically with lymphoscintigraphy (LS) and locall ...

1999

Fault Tolerance by Transparent Replication for Distributed Ada 95

This paper presents the foundations of Rapids ("Replicated Ada Partitions In Distributed Systems"), an implementation of the Distributed Systems Annex E incorporating the transparent replication of partitions in distributed Ada 95 applications. Rapids is a ...

Springer Verlag1999

Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses

Priscilla Turelli

The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measure the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcr ...

1996

Purification of telomerase from Euplotes aediculatus: requirement of a primer 3' overhang

Joachim Lingner

Telomerase is a ribonucleoprotein enzyme that uses its internal RNA moiety as a template for synthesis of telomeric repeats at chromosome ends. Here we report the purification of telomerase from Euplotes aediculatus by affinity chromatography with antisens ...

1996

Telomeres and the functional architecture of the nucleus

Thierry Laroche

The single molecule of DNA that constitutes a eukaryotic chromosome begins and ends with a stretch of repetitive DNA known as a telomere. These sequences appear to be necessary to preserve the integrity of the genetic material through the cell cycle.Telome ...

1993

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