Publications related to Immunostaining

Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics

Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz

The usage of microfluidics for automated and fast immunoassays has gained a lot of interest in the last decades. This integration comes with certain challenges, like the reconciliation of laminar flow patterns of micro-scale systems with diffusion-limited ...

ROYAL SOC CHEMISTRY2023

MALDI Detection of Exosomes: A Potential Tool for Cancer Studies

Hubert Girault, Horst Pick, Natalia Gasilova, Tzu-En Lin, Yingdi Zhu

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry is used to detect intact whole exosomes, yielding exosomal fingerprints within minutes. This rapid exosome detection approach is proposed as a potential tool for cancer studies. M ...

2019

Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections

Martinus Gijs, Daniel Migliozzi, Diego Gabriel Dupouy, Benjamin Pelz

Because of the close interaction between tumors and the immune system, immunotherapies are nowadays considered as the most promising treatment against cancer. In order to define the diagnosis and the subsequent therapy, crucial information about the immune ...

2019

Cell-based quantification of biomarkers from an ultra-fast microfluidic immunofluorescent staining: application to breast cancer cell lines

Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen

Immunohistochemistry (IHC) is one of the main techniques currently used in the clinics for biomarker characterization. It consists in colorimetric labeling with specific antibodies followed by microscopy analysis. The results are then used for diagnosis an ...

SPIE-INT SOC OPTICAL ENGINEERING2018

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Diego Gabriel Dupouy, Benjamin Pelz, Saska Brajkovic

BackgroundAnaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing f ...

BMC2018

Computational Immunohistochemistry: Recipes for Standardization of Immunostaining

Nuri Murat Arar, Maria Gabrani, Aditya Kashyap, Pushpak Pati, Anna Fomitcheva Khartchenko

Cancer diagnosis and personalized cancer treatment are heavily based on the visual assessment of immunohistochemically-stained tissue specimens. The precision of this assessment depends critically on the quality of immunostaining, which is governed by a nu ...

2017

Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections

Martinus Gijs, Diego Gabriel Dupouy, Saska Brajkovic

Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demons ...

Nature Publishing Group2017

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

Henning Paul-Julius Stahlberg, Anastasia Syntychaki

We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 320 nL of sample to prepare a cryo-EM grid, depending on the protocol used. The sample is applied and spread on the grid by a microcapillary. The procedure doe ...

Elsevier BV2017

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Immunostaining

Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms

Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics

Microscale physico-chemical interactions between hydrodynamically confined liquids and immersed biological surfaces

MALDI Detection of Exosomes: A Potential Tool for Cancer Studies

Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections

Cell-based quantification of biomarkers from an ultra-fast microfluidic immunofluorescent staining: application to breast cancer cell lines

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Computational Immunohistochemistry: Recipes for Standardization of Immunostaining

Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

Graph Chatbot

Chat with Graph Search

Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms

Microscale physico-chemical interactions between hydrodynamically confined liquids and immersed biological surfaces

Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics

MALDI Detection of Exosomes: A Potential Tool for Cancer Studies

Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Computational Immunohistochemistry: Recipes for Standardization of Immunostaining

Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections

Cell-based quantification of biomarkers from an ultra-fast microfluidic immunofluorescent staining: application to breast cancer cell lines

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts