Concept

Acetylcholine

Related publications (49)

Reversible sequential-binding probe receptor-ligand interactions in single cells

Horst Vogel, Rudolf Hovius, Jean-Manuel Segura, Marinela Gjoni Boillat, Christoph Schreiter

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

2005

Ganglion cells from chick retina display multiple functional nAChR subtypes

Mihai Adrian Ionescu, Philippe Renaud

We have examined the properties of nicotinic acetylcholine receptors in embryonic chick retinal ganglion cells. Ganglion cells, identified according to morphological and physiological criteria, displayed spontaneous or induced action potentials. In 94/99 c ...

2004

Downscaling Fourier Transform Infrared Spectroscopy to the Micrometer and Nanogram Scale: Secondary Structure of Serotonin and Acetylcholine Receptors

Horst Vogel, Rudolf Hovius, Horst Pick, Per Rigler, Erwin Ilegems

High signal-to-noise Fourier transform IR (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT3R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein ...

2003

Highly electrically insulating tethered lipid bilayers for probing the function of Ion channel proteins

Horst Vogel, Samuel Terrettaz, Michael Mayer

A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high elec. resistances. Elec. impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channe ...

2003

Selective immunolesions of CH4 cholinergic neurons do not disrupt spatial memory in rats

Tristan Bolmont

Adult male Long-Evans rats were subjected to bilateral lesions of the cholinergic neurons in the nucleus basalis magnocellularis (NBM) by injection of 0.2 or 0.4 mug 192-IgG-saporin in 0.4 mul phosphate-buffered saline, Control rats received an equivalent ...

Elsevier2002

Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes

Horst Vogel, Rudolf Hovius, Per Rigler

The nicotinic acetylcholine receptor from Torpedo was immobilised in tethered membranes. Surface plasmon resonance was used to quantify the binding of ligands and antibodies to the receptor. The orientation and structural integrity of the surface-reconstit ...

Elsevier2000

Ligand binding to nicotinic acetylcholine receptor investigated by surface plasmon resonance

Horst Vogel

Ligand binding to the nicotinic acetylcholine receptor is studied by surface plasmon resonance. Biotinylated bungarotoxin, immobilized on a streptavidin-coated gold film, binds nicotinic acetylcholine receptor both in detergent-solubilized and in lipid ves ...

1999

Supported lipid monolayers for the investigation of specific interactions between proteins and ligands

In this work, the specific and nonspecific binding of proteins to planar surfaces was investigated. The surfaces were first modified by the spontaneous adsorption of a number of synthesized 1,2-diacyl-sn-glycero-3-phosphatidylcholine lipids with different ...

EPFL1997

Combined effects of GDNF, BDNF, and CNTF on motoneuron differentiation in vitro

Patrick Aebischer

We have previously shown that glial cell line-derived neurotrophic factor (GDNF), in addition to promoting the survival of dopaminergic neurons in cultures from embryonic rat ventral mesencephalon,also increases the activity of choline acetyltransferase (C ...

1996

Quantitative determination of cross-relaxation rates in NMR using selective pulses to inhibit spin diffusion

Geoffrey Bodenhausen

Overhauser effects and hence internuclear distances can be measured accurately with selective expts. designed to suppress spin diffusion. It is essential to consider effects of both transverse and longitudinal relaxation during selective radiofrequency pul ...

1996

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