Concept
Third-generation sequencing
Related publications (147)
Multi-well plate lid for single-step pooling of 96 samples for high-throughput barcode-based sequencing
Bart Deplancke, Daniel Migliozzi, Gilles Weder, Riccardo Dainese, Daniel Alpern, Hüseyin Baris Atakan, Mustafa Demir, Dariia Gudkova
High-throughput transcriptomics is of increasing fundamental biological and clinical interest. The generation of molecular data from large collections of samples, such as biobanks and drug libraries, is boosting the development of new biomarkers and treatm ...
Dordrecht2024Comparison of Three Viral Nucleic Acid Preamplification Pipelines for Sewage Viral Metagenomics
Tamar Kohn, Xavier Fernandez Cassi
Viral metagenomics is a useful tool for detecting multiple human viruses in urban sewage. However, more refined protocols are required for its effective use in disease surveillance. In this study, we investigated the performance of three different preampli ...
2024Microbial genome collection of aerobic granular sludge cultivated in sequencing batch reactors using different carbon source mixtures
Christof Holliger, Aline Sondra Adler, Laetitia Janine Andrée Cardona, Jaspreet Singh Saini, Pilar Natalia Rodilla Ramírez, Ruizhe Pei
Aerobic granular sludge (AGS) consists of a microbial consortium that has an important role in wastewater treatment. This study investigates AGS microorganisms cultivated in a laboratory-scale sequencing batch reactor. Metagenomic sequencing was conducted ...
Amer Soc Microbiology2024Benchmarking informatics approaches for virus discovery: caution is needed when combining in silico identification methods
Understanding the ecological impacts of viruses on natural and engineered ecosystems relies on the accurate identification of viral sequences from community sequencing data. To maximize viral recovery from metagenomes, researchers frequently combine viral ...
Washington2024Genome sequences of Vibrio cholerae strains isolated in the DRC between 2009 and 2012
Melanie Blokesch, Sandrine Stutzmann, Alexandre Lemopoulos, Natalia Carolina Drebes Dorr
Vibrio cholerae has caused seven cholera pandemics in the past two centuries. The seventh and ongoing pandemic has been particularly severe on the African continent. Here, we report long read-based genome sequences of six V. cholerae strains isolated in th ...
2024Solid-State Nanopores for Biomolecular Analysis and Detection
Advances in nanopore technology and data processing have rendered DNA sequencing highly accessible, unlocking a new realm of biotechnological opportunities. Commercially available nanopores for DNA sequencing are of biological origin and have certain disad ...
Springer Link2024Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing.
Polymerase chain reaction (PCR) has been the most significant driver in the field of nucleic acid testing (NAT) since its invention. Popularized as an abbreviation by the Covid-19 pandemic, PCR-based methods are the gold standard in the field of diagnostic ...
EPFL2023