Publications related to Gel electrophoresis of proteins

Human PBMCs Form Lipid Droplets in Response to Spike Proteins

Intracellular lipid droplets (LDs) can accumulate in response to inflammation, metabolic stresses, and other physiological/pathological processes. Herein, we investigated whether spike proteins of SARS-CoV-2 induce LDs in human peripheral blood mononuclear ...

Basel2023

Molecular chaperones inject energy from ATP hydrolysis into the non-equilibrium stabilisation of native proteins

Paolo De Los Rios, Alessandro Barducci, Pierre Goloubinoff, Bruno Claude Daniel Fauvet, Alberto Stefano Sassi

Protein homeostasis, namely the ensemble of cellular mechanisms collectively controlling the activity, stability and conformational states of proteins, depends on energy-consuming processes. De novo protein synthesis requires ATP hydrolysis for peptide bon ...

2017

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

César Pulgarin, Sami Rtimi, Stefanos Giannakis, Cristina del Socorro Lonfat

In this work, the antimicrobial effects of the photo-Fenton process on yeast cells were tested (Saccharomyces cerevisiae), an essential eukaryotic unicellular model of living cells. Near-neutral pH was used in all studies, while iron sulfate and iron citra ...

Elsevier2016

Hyperpolarized Water to Study Protein-Ligand Interactions

Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Basile Vuichoud, Quentin Chappuis

The affinity between a chosen target protein and small molecules is a key aspect of drug discovery. Screening by popular NMR methods such as Water-LOGSY suffers from low sensitivity and from false positives caused by aggregated or denatured proteins. This ...

2015

Compatible Buffer for Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Hubert Girault, Natalia Gasilova, Liang Qiao, Hong-Xu Chen

A compatible buffer system for the coupling of capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed. The employed interface consists of a robot to drive a sliver-covered separation capil ...

Royal Society of Chemistry2013

A Novel Pulse-Chase SILAC Strategy Measures Changes in Protein Decay and Synthesis Rates Induced by Perturbation of Proteostasis with an Hsp90 Inhibitor

Vassily Hatzimanikatis, Julien Racle, Céline Hernández

Standard proteomics methods allow the relative quantitation of levels of thousands of proteins in two or more samples. While such methods are invaluable for defining the variations in protein concentrations which follow the perturbation of a biological sys ...

Public Library of Science2013

Segmented Field OFFGEL® Electrophoresis

Hubert Girault, Julien Maillard, Fernando Cortes Salazar, Dmitry Momotenko, Elena Tobolkina

A multielectrode setup for protein OFFGEL electrophoresis that significantly improves protein separation efficiency has been developed. Here, the electric field is applied by segments between seven electrodes connected in series to six independent power su ...

Wiley-Blackwell2012

High-sensitive protein analysis by FESI-CEMALDI-MS

Hubert Girault, Mohammad Reza Pourhaghighi

Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iont ...

Wiley-Blackwell2011

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Gel electrophoresis of proteins

Human PBMCs Form Lipid Droplets in Response to Spike Proteins

Telomere protection against oxidative stress

Molecular chaperones inject energy from ATP hydrolysis into the non-equilibrium stabilisation of native proteins

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

Hyperpolarized Water to Study Protein-Ligand Interactions

Compatible Buffer for Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A Novel Pulse-Chase SILAC Strategy Measures Changes in Protein Decay and Synthesis Rates Induced by Perturbation of Proteostasis with an Hsp90 Inhibitor

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Segmented Field OFFGEL® Electrophoresis

High-sensitive protein analysis by FESI-CEMALDI-MS

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Telomere protection against oxidative stress

Human PBMCs Form Lipid Droplets in Response to Spike Proteins

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

High-sensitive protein analysis by FESI-CEMALDI-MS

Hyperpolarized Water to Study Protein-Ligand Interactions

Compatible Buffer for Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A Novel Pulse-Chase SILAC Strategy Measures Changes in Protein Decay and Synthesis Rates Induced by Perturbation of Proteostasis with an Hsp90 Inhibitor

Molecular chaperones inject energy from ATP hydrolysis into the non-equilibrium stabilisation of native proteins

Segmented Field OFFGEL® Electrophoresis

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies