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A general method for covalent labeling of fusion proteins in vivo that complements the existing methods for protein labeling in vivo and in vitro is presented. This new method might lead to new ways of studying and manipulating proteins in living cells. Co ...
Max-Planck Institute, Hans-Knoll-Str. 8, D-07745 Jena, Germany. The cytotoxicity of several saturated and unsaturated marine diatom-derived aldehydes and an oxo-acid have been screened in vitro and in vivo against different organisms, such as bacteria, alg ...
Identification of a Strain Energy Function (SEF) is used when describing the complex mechanical properties of soft biological tissues such as the arterial wall. Classic SEFs, such as the one proposed by Chuong and Fung (J. Biomech. Eng. 105(3) (1983) 268), ...
The forced compression behavior of random 3D and 2D pulp fiber networks is investigated. Existing theories are found to describe well the evolution of packing stress with fiber volume fraction for the pulp networks. It was, moreover, found that the packing ...
Although phytoextraction using hyperaccumulating plants is seen as a promising technique, a lack of understanding of the basic physiological, biochemical, and molecular mechanisms involved in heavy metal hyperaccumulation prevents the optimization of the p ...
The effect of pH on Pb bioaccumulation by Chlorella kesslerii was studied. Both Pb uptake fluxes and Pb bound to membrane transport sites increased with an increase in pH from 4.0-5.0, were relatively stable in the pH interval 5.0-6.5, and increased again ...
A review of recent developments in the labeling of fusion proteins with synthetic mols. in the living cell or in complex mixts. The most commonly used tags are presented and then focuses on a new class of tags that are not limited to their genetically enco ...
Several washing agents ( EDTA, CDTA, NTA, 8 HQS, Ti-EDTA- citrate, Ca2+, and H+) were tested for their ability to extract adsorbed metal from biological surfaces to distinguish between internalized and total cellular metal (Pb, Ni, Cd, Zn, and Cu) for a gr ...
The specific and covalent labeling of fusion proteins with synthetic mols. opens up new ways to study protein function in the living cell. Here the authors present a novel method that allows for the specific and exclusive extracellular labeling of proteins ...
A new holographic technique combining digital holographic microscopy with the use of low coherence light has been developed and the potential of the resulting method for vision in turbid media and biomedical imaging has been evaluated. In digital holograph ...
