Concept
Thiol
Related publications (64)
Methods for high-throughput synthesis and screening of peptide libraries
Macrocycles provide an attractive modality for drug development but the identification of ligands to targets of interest is hindered by the lack of large macrocyclic compound libraries for high-throughput screening. A strategy to efficiently synthesize lar ...
EPFL2024Large Libraries of Structurally Diverse Macrocycles Suitable for Membrane Permeation
Christian Heinis, Xinjian Ji, Edward Jeffrey Will, Anne Sofie Luise Zarda, Alessandro Angelini, Alexander Lund Nielsen, Manuel Leonardo Merz, Mischa Schüttel, Ganesh Kumar Mothukuri, Zsolt Bognár
Macrocycles offer an attractive format for drug development due to their good binding properties and potential to cross cell membranes. To efficiently identify macrocyclic ligands for new targets, methods for the synthesis and screening of large combinator ...
Wiley-V C H Verlag Gmbh2024High-Density Immobilization of TCEP on Silica Beads for Efficient Disulfide Reduction and Thiol Alkylation in Peptides
Tris-(2-carboxyethyl)phosphine (TCEP) linked to agarose beads is widely used for reducing disulfide bridges in proteins and peptides. The immobilization of TCEP on beads allows efficient removal after reduction to prevent its reaction with alkylating reage ...
Weinheim2023Solid-phase peptide synthesis on disulfide-linker resin followed by reductive release affords pure thiol-functionalized peptides
Christian Heinis, Peter Matei Frisius Pânzar, Alexander Lund Nielsen, Manuel Leonardo Merz, Ganesh Kumar Mothukuri, Zsolt Bognár
Thiol groups are suitable handles for site-selectively modifying, immobilizing or cyclizing individual peptides or entire peptide libraries. A limiting step in producing the thiol-functionalized peptides is the chromatographic purification, which is partic ...
ROYAL SOC CHEMISTRY2022Controlling lignin solubility and hydrogenolysis selectivity by acetal-mediated functionalization
Jeremy Luterbacher, Anastasiia Komarova, Graham Reid Dick
Existing lignocellulosic biomass fractionation processes produce lignin with random, interunit C-C bonds that inhibit its depolymerization and constrain its use. Here, we exploit the aldehyde stabilization of lignin to tailor its structure, functionality, ...
ROYAL SOC CHEMISTRY2021The Effect of Thiol Structure on Allyl Sulfide Photodegradable Hydrogels and their Application as a Degradable Scaffold for Organoid Passaging
Matthias Lütolf, Nikolche Gjorevski
Intestinal organoids are useful in vitro models for basic and translational studies aimed at understanding and treating disease. However, their routine culture relies on animal-derived matrices that limit translation to clinical applications. In fact, ther ...
WILEY-V C H VERLAG GMBH2020Ethynylation of Cysteine Residues: From Peptides to Proteins in Vitro and in Living Cells
Jérôme Waser, Romain Gaëtan Tessier, Charlotte Marie Sornay, Raj Kumar Nandi
Current approaches to introduce terminal alkynes for bioorthogonal reactions into biomolecules still present limitations in terms of either reactivity, selectivity, or adduct stability. We present a method for the ethynylation of cysteine residues based on ...
2020Photocatalysis with organic dyes: facile access to reactive intermediates for synthesis
Jérôme Waser, Marion Marie-Agnès Garreau, Stephanie Grace Elizabeth Amos, Luca Buzzetti
Organic dyes have emerged as a reliable class of photoredox catalysts. Their great structural variety combined with the easy finetuning of their electronic properties has unlocked new possibilities for the generation of reactive intermediates. In this revi ...
2020Reversibly Cross-Linking Polymer Brushes Using Interchain Disulfide Bonds
The introduction of interchain cross-links in surface-grafted polymer brushes increases the robustness and mechanical properties of these thin polymer films. In most cases, cross-linked polymer brushes contain permanent interchain cross-links. The use of r ...
AMER CHEMICAL SOC2020Synthesis of DNA‐encoded disulfide‐ and thioether‐cyclized peptides
Christian Heinis, Van Manh Pham
DNA-encoded chemical library technologies enable the screening of large combinatorial libraries of chemically and structurally diverse molecules, including short cyclic peptides. A challenge in the combinatorial synthesis of cyclic peptides is the final st ...
2019