Concept
Electron tomography
Related publications (93)
Multidimensional Electron Imaging of Catalyst Layer Materials
The proton exchange membrane fuel cell (PEMFC) is an important technology for clean power generation in a decarbonized hydrogen system and is notably envisioned for applications in heavy-duty transportation. However, for cost and performance competitivenes ...
EPFL2023Correlating cryo-super resolution radial fluctuations and dual-axis cryo-scanning transmission electron tomography to bridge the light-electron resolution gap
Visualization of organelles and their interactions with other features in the native cell remains a challenge in modern biology. We have introduced cryo-scanning transmission electron tomography (CSTET), which can ac-cess 3D volumes on the scale of 1 micro ...
ACADEMIC PRESS INC ELSEVIER SCIENCE20233D reconstruction of curvilinear one-dimensional objects viewed in transmission electron microscopy
To obtain a more complete understanding of material microstructure at the nanoscale and to gain profound insights into their properties, there is a growing need for more efficient and precise methods that can streamline the process of 3D imaging using a tr ...
EPFL2023Towards near-atomic resolution imaging of microsecond nanoscale dynamics
Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...
EPFL2023Multi-electron Transfer by U(II) and Masked U(II) Complexes
Complexes of uranium in low oxidation state have shown the ability to activate non-reactive small molecules such as N-2. However, the multi-electron transfer required for such activation remains limited in uranium chemistry. Here, we review our recent rese ...
SWISS CHEMICAL SOC2022Random conical tilt reconstruction without particle picking in cryo-electron microscopy
A method is proposed to reconstruct the 3D molecular structure from micrographs collected at just one sample tilt angle in the random conical tilt scheme in cryo-electron microscopy. The method uses autocorrelation analysis on the micrographs to estimate f ...
INT UNION CRYSTALLOGRAPHY2022Waveguide modes spatially resolved by low-loss STEM-EELS
Duncan Thomas Lindsay Alexander
In an era of new developments in nanomaterials analysis enabled by the unprecedented spatial and energy resolutions of electron energy-loss spectroscopy in the scanning transmission electron microscope (STEM-EELS), it remains that the vast majority of work ...
2021Rapid melting and revitrification as an approach to microsecond time-resolved cryo-electron microscopy
Marcel Drabbels, Ulrich Lorenz, Pavel Olshin, Jonathan Mark Voss, Oliver Florian Harder
Proteins typically undergo dynamics on the microsecond to millisecond timescale, which is much faster than the time resolution of cryo-electron microscopy. Here, we propose a novel approach for microsecond time-resolved cryo-electron microscopy that involv ...
2021Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography
Davide Demurtas, Georgios Hatzopoulos, Sergey Nazarov, Alexandra Bezler, Paul Guichard, Veronika Nemcíková Villímová
Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...
2020Reconstruction Methods for Cryo-Electron Microscopy: From Model-based to Data-driven
The topic of this thesis is the development of new reconstruction methods for cryo-electron microscopy (cryo-EM). Cryo-EM has revolutionized the field of structural biology over the last decade and now permits the regular discovery of biostructures. Yet, t ...
EPFL2020