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Concept
Sulforhodamine B
Summary
Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.
The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light. It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.
Sulforhodamine B is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay).
Official source
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