Concept

Nucleic acid thermodynamics

Related publications (52)

Nucleic acid sequencing by processive degradation of dye-labeled polynucleotides immobilized on filter membranes

The invention relates to a method and a device for sequencing nucleic acid mols. on a perforated membrane. The inventive method and device can be esp. used in multiplex sequencing. The method involves prepg. an oligonucleotide that is labeled to satn. with ...

(Gnothis Holding SA, Switz.).2003

Hydrogen bonds lengths in nucleic acids estimated from longitudinal nitrogen-15 relaxation

Geoffrey Bodenhausen

A new NMR method has been designed for the measurement of the longitudinal relaxation rates of both donor and acceptor nitrogen-15 nuclei in Watson-Crick base pairs in 15N-enriched nucleic acids. The expt. was applied to a 22-nucleotide RNA hairpin. The le ...

2003

Nucleotide detection by fluorescence resonance energy transfer and methods thereof

Gerardo Turcatti

The present invention provides novel methods for detecting the incorporation of a nucleotide after a single base extension reaction. The methods make use of the Fluorescence Resonance Energy Transfer (FRET) effect generated by two fluorophores linked to th ...

2003

3'-Deoxyribopyranose (4' -> 2')-oligonucleotide duplexes (=p-DNA duplexes) as substitutes of RNA hairpin structures

Damian Ackermann

By automated synthesis, we prepd. hybrid oligonucleotides consisting of covalently linked RNA and p-DNA sequences (p-DNA = 3'-deoxyribopyranose (4'->2')-oligonucleotides). The pairing properties of corresponding hybrid duplexes formed from fully complement ...

2002

Inhibition of HIV-1 in cell culture by oligonucleotide-loaded nanoparticles

Didier Trono, Priscilla Turelli

PURPOSE: To investigate the potential use of polymeric nanoparticles for the delivery of antisense oligonucleotides in HIV-1-infected cell cultures. METHODS: Phosphorothioate oligonucleotides were encapsulated into poly (D,L-lactic acid) nanoparticles. Two ...

2001

Synthesis of Pyrrolidinone PNA: A Novel Conformationally Restricted PNA Analogue

To preorganize PNA for duplex formation, a new cyclic pyrrolidinone PNA analog has been designed. In this analog the aminoethyl-glycine backbone and the methylene-carbonyl linker are connected, introducing two chiral centers compared to PNA. The four stere ...

2001

Kinetics of collagen denaturation in tendon

Collagen is constituted of three polypeptides forming a triple helix maintained by numerous weak chemical interactions, such as hydrogen bonds. In connective tissue it further packs into very long cylinders of few nanometers of diameter, called fibrils. On ...

EPFL2000

Polarization-modulated FTIR spectroscopy of lipid/gramicidin monolayers at the air/water interface

Horst Vogel

Monolayers of gramicidin A, pure and in mixts. with dimyristoylphosphatidylcholine (DMPC), were studied in situ at the air/H2O and air/D2O interfaces by polarization-modulated IR reflection absorption spectroscopy (PM-IRRAS). Simulations of the entire set ...

1999

Design of Oligonucleotide Arrays at Interfaces

Horst Vogel

The surface attachment and detection of DNA probes are essential in the design of nucleic acid-based biosensors. A new strategy for the covalent immobilization of single-stranded oligonucleotides on gold-covered planar supports is presented. Optimization o ...

1999

Dispersive Hydrogen Diffusion in an Illuminated Undoped a-Si:H Film as a Function of Annealing Temperature

The dependence of the dispersion parameter α and of the time dependent diffusion parameter DH(tL) (estimated over a fixed diffusion length L) on annealing temperature Ta was investigated with elastic-recoil detection analysis (ERDA) in an undoped a-Si:H sa ...

1996

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