Concept

Ethics of cloning

Related publications (24)

Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics

Ratz et al. present an easy-to-use method to barcode progenitor cells, enabling profiling of cell phenotypes and clonal relations using single-cell and spatial transcriptomics, providing an integrated approach for understanding brain architecture. The mamm ...

NATURE PORTFOLIO2022

Traceable Impedance-Based Dispensing and Cloning of Living Single Cells

Philippe Renaud, Yann Barrandon, Ariane Rochat, David Vincent Bonzon, Stéphanie Claudinot, Georges Muller

Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using ...

2020

Information-theoretic limitations on approximate quantum cloning and broadcasting

Marius Christopher Lemm

We prove quantitative limitations on any approximate simultaneous cloning or broadcasting of mixed states. The results are based on information-theoretic (entropic) considerations and generalize the well-known no-cloning and no-broadcasting theorems. We al ...

2017

Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability

Julien Hiblot, Pierre Leblond

We describe here the construction of a new version of the Escherichia coli-Streptomyces shuttle cloning vector pIJ6902, which carries the original thiostrepton-inducible PtipA as well as the integrative and cloning systems, but with the E. coil low copy nu ...

Academic Press Inc Elsevier Science2015

A single epidermal stem cell strategy for safe ex vivo gene therapy

Graham Knott, Jacques Rougemont, Yann Barrandon, Ariane Rochat, Andrea Zaffalon, Nicolas Grasset, Stéphanie Danièle Hélène Droz-Georget, Sara Benmohammed, Emmanuelle Savioz-Dayer, Alessandra Recchia

There is a widespread agreement from patient and professional organisations alike that the safety of stem cell therapeutics is of paramount importance, particularly for ex vivo autologous gene therapy. Yet current technology makes it difficult to thoroughl ...

Wiley Open Access2015

Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture

Francesca Zagari

Mammalian cells represent the most widely used host system for the industrial production of recombinant therapeutic proteins. In order to increase productivity, chemically defined media are often used and optimized to provide the cells with the necessary n ...

EPFL2012

Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics

Sébastien Chenuet

The forthcoming arrival on the market of numerous protein therapeutics that require high clinical doses will foster the need for high-producing mammalian cell lines. Furthermore, pressures to reduce the development time for new therapeutics has meant more ...

EPFL2008

Thymic epithelial cells

Paola Bonfanti

As primary lymphoid organ, the thymus provides the essential environment for maturation and selection of functional T lymphocytes. Thymic epithelial (TE) cells derive from the endoderm, and more specifically, from the third pharyngeal pouch. The thymic epi ...

EPFL2008

Putative role of CG3160 in morphogen secretion

Gilles Udin

Morphogens are secreted molecules that influence the fate of neighboring cells in a concentration-dependant manner. For this reason, their distribution and diffusion strongly influence their roles. In my master thesis, I focused on a posttranslational modi ...

2008

Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability

Didier Trono

Conditionally immortalized human cells are valuable substrates for basic biologic studies, as well as for the production of specific proteins and for the creation of bioartificial organs. We previously demonstrated that the lentivector-mediated transductio ...

2003

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