Concept

Kinetochore

Related publications (127)

TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection

The shelterin proteins protect telomeres against activation of the DNA damage checkpoints and recombinational repair. We show here that a dimer of the shelterin subunit TRF2 wraps similar to 90 bp of DNA through several lysine and arginine residues localiz ...

Cell Press2016

Traceless Synthesis of Asymmetrically Modified Bivalent Nucleosomes

Beat Fierz, Carolin Christine Lechner, Ninad Dilip Agashe

Nucleosomes carry extensive post-translational modifications (PTMs), which results in complex modification patterns that are involved in epigenetic signaling. Although two copies of each histone coexist in a nucleosome, they may not carry the same PTMs and ...

Wiley-V C H Verlag Gmbh2016

Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies

Polo-like kinase 1 (PLK1) is a serine/threonine kinase that plays multiple and essential roles during the cell division cycle. Its inhibition in cultured cells leads to severe mitotic aberrancies and cell death. Whereas previous reports suggested that Plk1 ...

Wiley-Blackwell2016

Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN

Michaël Unser, Daniel Sage, Jacques Rougemont, Viesturs Simanis, Andrea Krapp, Daniel Andreas Schmitter, Anastasia Chasapi, Ioannis Xenarios

The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis ...

Company Of Biologists Ltd2015

An Extended, Boolean Model of the Septation Initiation Network in S-pombe Provides Insights into Its Regulation

Viesturs Simanis, Andrea Krapp, Elena Cano, Anastasia Chasapi, Ioannis Xenarios

Cytokinesis in fission yeast is controlled by the Septation Initiation Network (SIN), a protein kinase signaling network using the spindle pole body as scaffold. In order to describe the qualitative behavior of the system and predict unknown mutant behavio ...

Public Library of Science2015

Pombe's thirteen - control of fission yeast cell division by the septation initiation network

Viesturs Simanis

The septation initiation network (SIN) regulates aspects of cell growth and division in Schizosaccharomyces pombe and is essential for cytokinesis. Insufficient signalling results in improper assembly of the contractile ring and failure of cytokinesis, gen ...

Company Of Biologists Ltd2015

Purification of centrosomes from mammalian cell lines

Paul Guichard

Centrosomes act as the main microtubule-organizing centre of animal cells and play critical roles in the cell, such as mitotic spindle organization, cell polarity, and motility. They are composed of two barrel-shaped structures, the centrioles, surrounded ...

Elsevier Academic Press Inc2015

Clathrin regulates centrosome positioning by promoting acto-myosin cortical tension in C. elegans embryos

Pierre Gönczy, Alessandro De Simone, Sylvain Träger, Kalyani Thyagarajan

Regulation of centrosome and spindle positioning is crucial for spatial cell division control. The one-cell Caenorhabditis elegans embryo has proven attractive for dissecting the mechanisms underlying centrosome and spindle positioning in a metazoan organi ...

Company Of Biologists Ltd2014

Mechanisms of differential centriole inheritance in C. elegans

Lukas von Tobel

Centrosomes are the principal microtubule organizing center (MTOC) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated to ensure bipolar spindle formation and thus fait ...

EPFL2014

Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis

Viesturs Simanis

Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, bu ...

Royal Society, The2014

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