Genetic Mechanisms: Prader Willi Syndrome

This lecture discusses the genetic mechanisms behind Prader Willi syndrome, a rare genetic disorder with a prevalence of about 1 in 50,000 newborns. The instructor explains the deletion of a specific region on chromosome 15, leading to various symptoms such as motor skill impairments and high pain tolerance. The use of restriction enzymes like Mst II in DNA digestion is also covered, along with Southern blotting techniques to analyze DNA fragments. The process of electrophoresis to separate DNA fragments by size and the hybridization of labeled DNA probes for detection are explained in detail.