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This lecture covers the translation process in eukaryotes, focusing on the role of transfer RNA (tRNA) in carrying amino acids, the structure of ribosomes, and the accuracy of translation ensured by aminoacyl-tRNA synthetases. It explains the initiation, elongation, and termination steps of translation, detailing how ribosomes add amino acids, form peptide bonds, and translocate along the mRNA. The lecture also discusses the importance of energy in the translation process and the mechanism of translocation. Additionally, it explores the monocistronic nature of eukaryotic messengers and the function of multiple ribosomes on a single mRNA.