This lecture covers the principles of Nuclear Magnetic Resonance (NMR) spectroscopy, explaining the energy splitting in a magnetic field and the resonance condition. It also discusses the applications of NMR in determining chemical structures and kinetics. Additionally, Surface Plasmon Resonance (SPR) is explored, detailing how it works, its applications in biosensing, and the setup of an SPR instrument. The lecture delves into SPR imaging, ligand binding, and the Biacore 3000 system for high-performance research, emphasizing its sensitivity and advanced kinetic evaluation capabilities.