This lecture by the instructor focuses on quantitative single-protein imaging to reveal the molecular complex formation of integrin, talin, and kindlin during cell adhesion. The presentation covers topics such as DNA-PAINT simulations, talin nanoscale organization, and spatial association of talin and kindlin. The speaker also discusses the quantification of molecules in clusters and the spatial organization of proteins at the nanoscale. Acknowledgments are given to various contributors to the Super Resolution Project.