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This lecture covers the thermodynamic retention principles for a packed column in gas chromatography, focusing on the interaction between gases, the retention of solutes, and the calculation of distribution constants. It also discusses the van Deemter equation for different column types, the impact of temperature on separation, the efficiency of stationary phases, and the various detectors used in gas chromatography. The importance of temperature programming, stationary phase modifications, and the analysis of standard compounds are also highlighted.