Introduces optical methods in chemistry, covering ray optics, lasers, spectroscopy, and X-ray physics, emphasizing light-matter interactions and Nobel Prize-winning advancements.
Explores the interaction of light with molecules, including exciton formation, absorption and emission spectra, and selection rules for electronic transitions.
Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.