Electron Microscopy: ESEM

This lecture covers the Electron Scanning Electron Microscopy (ESEM) technique, which allows the study of samples in various environments such as gas or liquid. It explains the differential pumping system used to maintain pressure gradients, the benefits of reducing diffusion length, and the detection of ionized gas particles. The ESEM technique enables imaging of wet, non-conductive, and dirty samples, as well as the observation of phase transitions and oxidation processes. Additionally, it discusses the challenges of imaging living organisms and hydrated objects. The lecture emphasizes the importance of controlling pressure, temperature, and humidity to avoid condensation and achieve high-quality images.