Surface Sensors: Binding Kinetics and Langmuir Isotherm

This lecture covers the array of surface sensors used for detecting molecules by affinity, discussing main features like surface-sensing of molecules, multiple probes, and small sample volume. It also delves into binding and dissociation kinetics on the surface, explaining the association, dissociation, and regeneration processes. The Langmuir binding isotherm is explored in detail, focusing on concentration, density of binding sites, affinity constant, and dissociation constant. Additionally, the lecture addresses adsorption kinetics, the concentration of analyte, and the total amount of analyte in an array. It concludes with strategies to overcome issues related to probe density and volume of the incubation chamber.