Biophotonics Fundamentals

This lecture covers the fundamentals of biophotonics, focusing on the Franck-Condon rule, absorption/transmittance, Beer-Lambert Law, fluorescence, phosphorescence, and the principles of spectroscopy. It explains the influence of energy level populations, temperature, and energy level spacing on absorption. The Franck-Condon principle is discussed in relation to electronic transitions and vibrational states. The lecture also delves into the quantum yield of fluorescence, fluorescence intensity, and the role of chromophores and fluorophores in biological systems. Additionally, it explores the absorption and emission spectra of biological chromophores, nucleic acids, and lipids, as well as the use of NADH and FAD as metabolic indicators.