Measurements: ConsO2

In courses (2)

Description

This lecture covers the process of measuring cell consumption in a controlled environment, including cleaning the chamber, degassing the solution, preparing cell samples, and conducting measurements to analyze oxygen consumption. The instructor demonstrates the importance of accurate pipetting and temperature control to ensure reliable results.

Instructor

Official source

https://mediaspace.epfl.ch/media/0_aqjnoamy

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Related concepts (4)

Degassing

Degassing, also known as degasification, is the removal of dissolved gases from liquids, especially water or aqueous solutions. There are numerous methods for removing gases from liquids. Gases are removed for various reasons. Chemists remove gases from solvents when the compounds they are working on are possibly air- or oxygen-sensitive (air-free technique), or when bubble formation at solid-liquid interfaces becomes a problem. The formation of gas bubbles when a liquid is frozen can also be undesirable, necessitating degassing beforehand.

Oxygen

Oxygen is the chemical element with the symbol O and atomic number 8. It is a member of the chalcogen group in the periodic table, a highly reactive nonmetal, and an oxidizing agent that readily forms oxides with most elements as well as with other compounds. Oxygen is Earth's most abundant element, and after hydrogen and helium, it is the third-most abundant element in the universe. At standard temperature and pressure, two atoms of the element bind to form dioxygen, a colorless and odorless diatomic gas with the formula O2.

Blood

Blood is a body fluid in the circulatory system of humans and other vertebrates that delivers necessary substances such as nutrients and oxygen to the cells, and transports metabolic waste products away from those same cells. Blood in the circulatory system is also known as peripheral blood, and the blood cells it carries, peripheral blood cells. Blood is composed of blood cells suspended in blood plasma.

Oxygen sensor

An oxygen sensor (or lambda sensor, where lambda refers to air–fuel equivalence ratio, usually denoted by λ) or probe or sond, is an electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analysed. It was developed by Robert Bosch GmbH during the late 1960s under the supervision of Dr. Günter Bauman. The original sensing element is made with a thimble-shaped zirconia ceramic coated on both the exhaust and reference sides with a thin layer of platinum and comes in both heated and unheated forms.