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This lecture covers the application of DNA technology, focusing on PCR as a method to amplify DNA sequences. It explains the process of PCR, its significance in genetic analysis, and how it is used in forensic science. Additionally, it discusses genetic diversity between individuals, highlighting SNPs and microsatellites as key markers. The lecture also delves into the molecular mechanisms behind microsatellite variations and their analysis using PCR techniques. Furthermore, it explores the use of DNA profiling in paternity tests and forensic investigations, emphasizing the uniqueness of DNA profiles. Lastly, it touches on the detection of genetic disorders like trisomy 21 and the implications of maternal age on genetic conditions.