Probing Translation Dynamics: Ribosome Profiling & ER Engagement

This lecture delves into the intricacies of translation dynamics, focusing on ribosome profiling and ER engagement. The instructor discusses the spatial and temporal aspects of translation in prokaryotic and eukaryotic cells, emphasizing the differences in transcription and translation processes. The lecture explores the architecture of mammalian cells, the localization of translation, and the role of ribosomes in protein synthesis. Advanced concepts such as polysome fractionation, ribosome profiling, and biotin-streptavidin binding are also covered. The lecture concludes with a discussion on the dynamics of ER-associated ribosomes and the implications for protein trafficking and sorting.