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This lecture covers the characterisation and performance of biomaterials, focusing on hydrogels. It includes an overview of materials used in biomaterials, such as naturally derived and manmade biomaterials, polymers, nanoparticles, and surfaces. The lecture also delves into specific applications like materials for drug delivery, cell adhesion, immune engineering, and tissue engineering. The measurement, regulation, and translation of biomaterial properties are discussed, along with topics like sensors, regulatory aspects, and trials. Various techniques for characterising biomaterial properties, including stiffness, degradation, swelling, and biocompatibility, are explored through methodologies like rheology, microscopy, and in vitro degradation experiments.