This lecture presents the LiveSense project, focusing on developing autonomous cell-based biosensors for environmental sensing and toxicology. The project involves collaborations to create an integrated system with various measurement methods, including impedance and fluorescence detection. The lecture covers the challenges of using mammalian and genetically modified bacterial cells, as well as the development of microfluidic systems for continuous monitoring. The project aims to demonstrate the feasibility of deploying these biosensors in the field, showcasing the potential for real-time toxicity screening and environmental monitoring.